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Invitrogen

p27 Kip1 Polyclonal Antibody

Advanced Verification
1 Published Figure
8 References
View all (86) p27Kip1 antibodies

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Product Details

PA5-27188

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 2 publications 2 publications

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.07 mg/mL

Amount

7 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544664

Product Specific Information

Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; P27 CDKN1B; p27(KIP1); p27Kip1; unnamed protein product

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Gene Aliases: AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

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UniProt ID: (Human) P46527, (Mouse) P46414

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Entrez Gene ID: (Human) 1027, (Mouse) 12576, (Rat) 83571

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Function(s)
protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding protein kinase binding protein phosphatase binding cyclin-dependent protein kinase activating kinase regulator activity cyclin binding ubiquitin protein ligase binding macromolecular complex binding chaperone binding binding, bridging molecular function inhibitor activity ubiquitin ligase activator activity cysteine-type endopeptidase activator activity involved in apoptotic process Hsp70 protein binding protein complex binding kinase activity kinase inhibitor
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle placenta development potassium ion transport apoptotic process regulation of exit from mitosis Notch signaling pathway regulation of mitotic cell cycle heart development sensory perception of sound positive regulation of cell proliferation negative regulation of cell proliferation programmed cell death negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator regulation of cell migration positive regulation of microtubule polymerization negative regulation of kinase activity positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of DNA replication negative regulation of transcription, DNA-templated negative regulation of growth negative regulation of mitotic cell cycle autophagic cell death inner ear development negative regulation of epithelial cell proliferation nuclear export regulation of cell cycle epithelial cell proliferation involved in prostate gland development negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to antibiotic cellular response to lithium ion cellular senescence regulation of mitotic cell cycle phase transition regulation of lens fiber cell differentiation regulation of cell cycle G1/S phase transition epithelial cell apoptotic process negative regulation of cyclin-dependent protein kinase activity negative regulation of epithelial cell apoptotic process negative regulation of vascular smooth muscle cell proliferation negative regulation of cardiac muscle tissue regeneration regulation of G1/S transition of mitotic cell cycle activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle cell cycle arrest cell death positive regulation of cell death regulation of cell proliferation negative regulation of phosphorylation negative regulation of apoptotic process negative regulation of cellular component movement cellular response to organic cyclic compound mitotic cell cycle arrest response to hypoxia response to glucose response to organic cyclic compound phosphorylation response to estradiol response to drug response to amino acid response to peptide hormone response to cadmium ion
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