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          • Primary Antibodies ›
          • IGF1 Antibodies
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          Invitrogen

          IGF1 Polyclonal Antibody

          3 Published Figures
          6 References
          View all (39) IGF1 antibodies

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          Cite IGF1 Polyclonal Antibody

          Additional Information:
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          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (3)
          • Published Figures (3)
          IGF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          IGF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          IGF1 Antibody (PA5-27207) in ICC/IF

          IGF1 Polyclonal Antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 Polyclonal Antibody (Product # PA5-27207) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          IGF1 Antibody in Immunocytochemistry (ICC/IF)
          IGF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Immunohistochemistry (IHC)
          IGF1 Antibody in Western Blot (WB)
          IGF1 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-27207

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chordate, Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.16 mg/mL

          Amount

          116 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2544683

          Product Specific Information

          Recommended positive controls: PC-3, U87-MG, SK-N-SH.

          Predicted reactivity: Mouse (93%), Rat (94%), Xenopus laevis (80%), Dog (98%), Pig (96%), Rabbit (98%), Chicken (85%), Sheep (96%), Rhesus Monkey (100%), Bovine (97%), Guinea pig (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: H-IGF-1; IGF; IGF-1; Igf-1 Ea; Igf-I Eb; IGF-IA; IGF-IB; IGF1; IGF1A; IGFIa; Insulin like growth factor; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I precursor; insulin-like growth factor IB; insulin-like growth factor IB prepropeptide; insulin-like growth factor precursor; M-IGF-1; mechano growth factor; OTTHUMP00000195084; R-IGF-1; somatomedin; Somatomedin C; somatomedin-C; unnamed protein product

          View more View less

          Gene Aliases: C730016P09Rik; IGF; Igf-1; IGF-I; IGFI; MGF

          View more View less

          UniProt ID: (Human) P05019, (Mouse) P05017

          View more View less

          Entrez Gene ID: (Human) 3479, (Mouse) 16000

          View more View less

          Function(s)
          insulin receptor binding insulin-like growth factor receptor binding integrin binding hormone activity protein binding growth factor activity receptor agonist activity steroid binding transmembrane receptor protein tyrosine kinase activator activity protein serine/threonine kinase activator activity
          Process(es)
          skeletal system development osteoblast differentiation cell activation epithelial to mesenchymal transition signal transduction Ras protein signal transduction muscle organ development circadian rhythm cell proliferation positive regulation of cell proliferation insulin receptor signaling pathway response to heat glycolate metabolic process regulation of gene expression positive regulation of glycoprotein biosynthetic process positive regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration muscle hypertrophy myotube cell development positive regulation of smooth muscle cell migration proteoglycan biosynthetic process positive regulation of cell migration positive regulation of myelination activation of protein kinase B activity negative regulation of interleukin-1 beta production negative regulation of tumor necrosis factor production negative regulation of smooth muscle cell apoptotic process bone mineralization involved in bone maturation wound healing positive regulation of activated T cell proliferation negative regulation of apoptotic process positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of skeletal muscle tissue regeneration positive regulation of insulin-like growth factor receptor signaling pathway myoblast differentiation positive regulation of osteoblast differentiation positive regulation of glycogen biosynthetic process positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of Ras protein signal transduction insulin-like growth factor receptor signaling pathway positive regulation of fibroblast proliferation positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of protein secretion protein stabilization myoblast proliferation positive regulation of protein kinase B signaling negative regulation of oocyte development growth hormone receptor signaling pathway positive regulation of cell growth involved in cardiac muscle cell development positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade negative regulation of release of cytochrome c from mitochondria receptor signaling pathway via STAT postsynaptic modulation of chemical synaptic transmission negative regulation of neuroinflammatory response negative regulation of beta-amyloid formation positive regulation of trophectodermal cell proliferation cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell apoptotic process positive regulation of transcription regulatory region DNA binding negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway regulation of protein phosphorylation blood vessel remodeling negative regulation of immune system process regulation of translation nervous system development sensory perception sensory perception of smell memory negative regulation of cell proliferation glial cell differentiation negative regulation of muscle cell apoptotic process myotube differentiation positive regulation of cerebellar granule cell precursor proliferation positive regulation of cell growth lung development mammary gland development exocrine pancreas development regulation of establishment or maintenance of cell polarity multicellular organism growth swimming behavior regulation of cell proliferation regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration regulation of nitric oxide biosynthetic process response to ethanol positive regulation of fat cell differentiation lung alveolus development cell development branching morphogenesis of an epithelial tube inner ear development chondroitin sulfate proteoglycan biosynthetic process negative regulation of peptidyl-tyrosine phosphorylation detection of mechanical stimulus involved in sensory perception regulation of protein metabolic process regulation of calcium ion transport positive regulation of glial cell proliferation lung vasculature development lung lobe morphogenesis Type I pneumocyte differentiation Type II pneumocyte differentiation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis prostate gland growth prostate gland epithelium morphogenesis prostate gland stromal morphogenesis negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade cellular response to glucose stimulus positive regulation of steroid hormone biosynthetic process extrinsic apoptotic signaling pathway in absence of ligand negative regulation of oligodendrocyte apoptotic process negative regulation of cholangiocyte apoptotic process positive regulation of vascular endothelial cell proliferation retinal cell apoptotic process cellular response to insulin-like growth factor stimulus positive regulation of myoblast proliferation
          It has to be done as per old AB suggested Products section.

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