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Invitrogen
VHL Polyclonal Antibody
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FIGURE: 1 / 10
VHL Antibody (PA5-27322) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of VHL was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VHL Polyclonal Antibody (Product # PA5-27322) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Product Details
PA5-27322
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Myc-DDK-tagged VHL-transfected 293T.
Predicted reactivity: Mouse (93%), Rat (91%), Dog (95%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: elongin binding protein; Protein G7; pVHL; unnamed protein product; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1; Vhlh
UniProt ID: (Human) P40337, (Mouse) P40338
Entrez Gene ID: (Human) 7428, (Mouse) 22346
obsolete transcription elongation regulator activity
ubiquitin-protein transferase activity
protein binding
enzyme binding
binding, bridging
protein serine/threonine kinase binding
DNA-binding transcription factor binding
protein binding, bridging involved in substrate recognition for ubiquitination
transcription factor binding
protein complex binding
ubiquitin protein ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
response to hypoxia
regulation of transcription, DNA-templated
proteolysis
negative regulation of cell proliferation
negative regulation of signal transduction
regulation of gene expression
negative regulation of autophagy
negative regulation of macromolecule biosynthetic process
negative regulation of gene expression
protein ubiquitination
negative regulation of transcription elongation from RNA polymerase II promoter
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of cell differentiation
positive regulation of transcription, DNA-templated
negative regulation of JAK-STAT cascade
protein stabilization
cellular response to hypoxia
regulation of cellular response to hypoxia
negative regulation of TORC1 signaling
amyloid fibril formation
angiogenesis
type B pancreatic cell differentiation
pancreatic A cell differentiation
NLS-bearing protein import into nucleus
proteasomal protein catabolic process
protein transport
protein catabolic process
neuron differentiation
extracellular matrix organization
regulation of catecholamine metabolic process
blood vessel endothelial cell migration
negative regulation of endothelial cell differentiation
protein heterooligomerization
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
regulation of thymocyte apoptotic process
negative regulation of thymocyte apoptotic process
negative regulation of hypoxia-inducible factor-1alpha signaling pathway
regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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