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          • Primary Antibodies ›
          • HMGB1 Antibodies

          Invitrogen

          HMGB1 Polyclonal Antibody

          Advanced Verification
          1 Published Figure
          5 References
          View all (59) HMGB1 antibodies

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          Cite HMGB1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (13)
          • Published Figures (1)
          • Advanced Verification (2)
          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
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          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          HMGB1 Antibody (PA5-27378) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of HMGB1 was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 Polyclonal Antibody (Product # PA5-27378) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody
          HMGB1 Antibody

          Product Details

          PA5-27378

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunohistochemistry - Free Floating (IHC (Free))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat, Zebrafish

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.43 mg/mL

          Amount

          43 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 1% BSA, 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2544854

          Product Specific Information

          Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, C2C12, Raw264.7, PC-12, HMGB1-transfected 293T.

          Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; non-histone protein HMG1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

          View more View less

          Gene Aliases: Ac2-008; amphoterin; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

          View more View less

          UniProt ID: (Human) P09429, (Pig) P12682, (Rat) P63159, (Mouse) P63158

          View more View less

          Entrez Gene ID: (Human) 3146, (Pig) 445521, (Rat) 25459, (Mouse) 15289

          View more View less

          Function(s)
          four-way junction DNA binding bubble DNA binding transcription regulatory region sequence-specific DNA binding lipopolysaccharide binding phosphatidylserine binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity transcription corepressor activity RNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity integrin binding protein binding lipid binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity receptor agonist activity RAGE receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA polymerase binding supercoiled DNA binding DNA-binding transcription factor binding non-sequence-specific DNA binding, bending open form four-way junction DNA binding crossed form four-way junction DNA binding bent DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding 5S rRNA binding transcription factor binding heparin binding peptide binding poly(A) RNA binding protein dimerization activity glycolipid binding repressing transcription factor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter eye development myeloid dendritic cell activation endothelial cell proliferation positive regulation of endothelial cell proliferation activation of innate immune response adaptive immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response myeloid progenitor cell differentiation immune system process dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell glycogen catabolic process DNA metabolic process DNA topological change DNA repair base-excision repair double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination chromatin organization chromatin remodeling transcription from RNA polymerase II promoter autophagy chemotaxis inflammatory response immune response cellular response to DNA damage stimulus signal transduction positive regulation of cytosolic calcium ion concentration positive regulation of autophagy negative regulation of endothelial cell migration negative regulation of RNA polymerase II transcriptional preinitiation complex assembly myeloid cell differentiation lung development neuron projection development heterochromatin assembly regulation of restriction endodeoxyribonuclease activity DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation innate immune response positive regulation of innate immune response positive regulation of cell differentiation positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of viral entry into host cell cell development regulation of viral process positive chemotaxis regulation of DNA metabolic process response to glucocorticoid positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide positive regulation of monocyte chemotactic protein-1 production positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance cellular response to interleukin-7 positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis regulation of hemopoiesis positive regulation of myeloid progenitor cell differentiation positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of apoptotic cell clearance regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation cell morphogenesis positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation base-excision repair, DNA ligation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter nervous system development circadian rhythm negative regulation of DNA replication positive regulation of cell proliferation response to heat response to glucose positive regulation of cell death positive regulation of neuron projection development positive regulation of smooth muscle cell migration positive regulation of cell migration actin cytoskeleton reorganization response to lipopolysaccharide response to insulin positive regulation of myeloid cell apoptotic process response to interferon-gamma response to drug positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of myoblast differentiation positive regulation of protein kinase activity positive regulation of mitotic cell cycle regulation of inflammatory response male-specific defense response to bacterium induction of positive chemotaxis myoblast proliferation cellular response to interleukin-1 MyD88-dependent toll-like receptor signaling pathway nucleosome assembly toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway DNA ligation involved in DNA repair positive regulation of DNA ligation positive regulation of DNA ligase activity
          It has to be done as per old AB suggested Products section.

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