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Invitrogen

ASK1 Polyclonal Antibody

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ASK1 Polyclonal Antibody

Product Details

PA5-28692

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ASK1. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546168

Product Specific Information

Recommended positive controls: HeLaS3, mouse lung, MAP3K5-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (91%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4; unnamed protein product

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Gene Aliases: 7420452D20Rik; ASK; ASK1; MAP3K5; MAPKKK5; MEKK5

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UniProt ID: (Human) Q99683, (Mouse) O35099

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Entrez Gene ID: (Human) 4217, (Mouse) 26408

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Function(s)
nucleotide binding magnesium ion binding protein kinase activity protein serine/threonine kinase activity JUN kinase kinase kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding protein phosphatase binding protein domain specific binding identical protein binding protein homodimerization activity metal ion binding protein serine kinase activity cysteine-type endopeptidase activator activity involved in apoptotic process transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade immune system process response to ischemia apoptotic process JNK cascade intrinsic apoptotic signaling pathway in response to oxidative stress positive regulation of cardiac muscle cell apoptotic process cellular response to amino acid starvation response to endoplasmic reticulum stress neuron intrinsic apoptotic signaling pathway in response to oxidative stress p38MAPK cascade positive regulation of apoptotic process regulation of programmed cell death positive regulation of MAPK cascade innate immune response positive regulation of myoblast differentiation positive regulation of transcription, DNA-templated positive regulation of JNK cascade neuron apoptotic process stress-activated MAPK cascade intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to tumor necrosis factor endothelial cell apoptotic process cellular senescence apoptotic signaling pathway programmed necrotic cell death positive regulation of p38MAPK cascade cellular response to reactive nitrogen species positive regulation of vascular smooth muscle cell proliferation activation of MAPKK activity activation of MAPK activity positive regulation of protein phosphorylation protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cell death phosphorylation positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of JUN kinase activity positive regulation of neuron death
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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