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Invitrogen
P-Glycoprotein Polyclonal Antibody
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FIGURE: 1 / 8
P-Glycoprotein Antibody (PA5-28801) in WB
Western blot was performed using Anti-P-Glycoprotein Polyclonal Antibody (Product # PA5-28801) and due to glycosylation, 160-250 kDa band corresponding to ABCB1 was observed across cell lines tested except MCF-7 which is reported to be negative. Whole cell extracts (30 µg lysate) of HepG2 (Lane 1), Caco-2 (Lane 2) and MCF-7 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell SureLock™ Mini-Cell and XC... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-28801
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human P Glycoprotein. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.58 mg/mL
Amount
158 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HepG2, HepG2 membrane fraction extract, unboild HepG2, unboiled HepG2 membrane fraction extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-binding cassette, sub-family B (MDR/TAP), member 1; colchicin sensitivity; doxorubicin resistance; MDR1; P-glycoprotein; multidrug resistance protein 1; multidrug-resistance; P-glycoprotein; P-glycoprotein 1; phospholipid transporter ABCB1; unnamed protein product; uORF
Gene Aliases: ABC20; CD243; CLCS; ENPAT; GP170; MDR1; p-170; P-GP; PGY1
UniProt ID: (Human) P08183
Entrez Gene ID: (Human) 5243
protein binding
ATP binding
xenobiotic-transporting ATPase activity
efflux transmembrane transporter activity
ATPase activity
transmembrane transporter activity
ubiquitin protein ligase binding
ATPase activity, coupled to transmembrane movement of substances
xenobiotic transporter activity
carboxylic acid transmembrane transporter activity
phosphatidylcholine-translocating ATPase activity
phosphatidylethanolamine flippase activity
ceramide floppase activity
ATPase-coupled intramembrane lipid transporter activity
floppase activity
phosphatidylethanolamine floppase activity
ABC-type transporter activity
G2/M transition of mitotic cell cycle
xenobiotic metabolic process
response to xenobiotic stimulus
phospholipid translocation
terpenoid transport
transmembrane transport
transepithelial transport
stem cell proliferation
ceramide translocation
export across plasma membrane
transport across blood-brain barrier
carboxylic acid transmembrane transport
drug transmembrane export
drug transport across blood-brain barrier
regulation of chloride transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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