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Invitrogen
Calpain 2 Polyclonal Antibody
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FIGURE: 1 / 6
Calpain 2 Antibody (PA5-29381) in IHC (P)
Calpain 2 Polyclonal Antibody detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29381
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.47 mg/mL
Amount
47 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, mouse brain.
Predicted reactivity: Mouse (91%), Rat (91%), Pig (94%), Rabbit (93%), Chicken (86%), Sheep (94%), Rhesus Monkey (98%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 80 kDa M-calpain subunit; Ca2-activated neutral protease subunit; calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain M-type; calpain, large polypeptide L2; calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; millimolar-calpain; unnamed protein product
Gene Aliases: CALP80; CANP2; CANPL2; CANPml; Capa-2; Capa2; m-calpain; m-calpin; mCANP
UniProt ID: (Human) P17655, (Mouse) O08529
Entrez Gene ID: (Human) 824, (Mouse) 12334
response to hypoxia
proteolysis
female pregnancy
response to mechanical stimulus
positive regulation of cardiac muscle cell apoptotic process
protein autoprocessing
protein catabolic process
regulation of interleukin-6 production
cellular response to interferon-beta
response to hydrogen peroxide
behavioral response to pain
synaptic vesicle endocytosis
regulation of cytoskeleton organization
negative regulation of focal adhesion assembly
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
vascular endothelial cell response to laminar fluid shear stress
vascular endothelial cell response to oscillatory fluid shear stress
protein catabolic process at postsynapse
positive regulation of myoblast fusion
positive regulation of phosphatidylcholine biosynthetic process
blastocyst development
myoblast fusion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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