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Invitrogen
ChAT Polyclonal Antibody
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FIGURE: 1 / 11
ChAT Antibody (PA5-29653) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ChAT was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-29653
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.15 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T.
Predicted reactivity: Mouse (93%), Rat (92%), Pig (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; Choline O-acetyltransferase
Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6
UniProt ID: (Human) P28329
Entrez Gene ID: (Human) 1103, (Rat) 290567, (Mouse) 12647
phosphatidylcholine biosynthetic process
neurotransmitter transport
neuromuscular synaptic transmission
acetylcholine biosynthetic process
antral ovarian follicle growth
response to hypoxia
chemical synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
response to nutrient
memory
rhythmic behavior
adult walking behavior
metabolic process
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
response to drug
rhythmic excitation
response to ethanol
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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