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Invitrogen
GTF2B Polyclonal Antibody
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FIGURE: 1 / 1
GTF2B Antibody (PA5-29812) in WB
Western blot analysis of GTF2B in various whole cell extracts (30 µg). Samples were incubated with GTF2B polyclonal antibody (Product # PA5-29812) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 12% SDS-PAGE. Competitors antibody diluted at 1:200.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29812
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.55 mg/mL
Amount
55 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, HeLa nuclear extract, Jurkat, Raji, NCI-H929.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (97%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: general transcription factor TFIIB; RNA polymerase II transcription factor IIB; RP11-82K18.1; S300-II; transcription factor; unnamed protein product
Gene Aliases: TF2B; TFIIB
UniProt ID: (Human) Q00403
Entrez Gene ID: (Human) 2959
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II core binding
DNA binding
protein binding
zinc ion binding
obsolete general RNA polymerase II transcription factor activity
acetyltransferase activity
TBP-class protein binding
thyroid hormone receptor binding
peptide-lysine-N-acetyltransferase activity
DNA-binding transcription factor binding
promoter-specific chromatin binding
transcriptional start site selection at RNA polymerase II promoter
DNA-templated transcription, initiation
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
protein acetylation
gene expression
viral transcription
RNA polymerase II transcriptional preinitiation complex assembly
meiotic sister chromatid cohesion
spindle assembly
chromosome organization
transcription preinitiation complex assembly
positive regulation of core promoter binding
RNA Polymerase II core complex assembly
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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