Search
Invitrogen
GNAO1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 13
GNAO1 Antibody (PA5-30044) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GNAO1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNAO1 Polyclonal Antibody (Product # PA5-30044) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-30044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, mouse lung, rat brain, rat lung.
Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (98%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp686O0962; G alpha o; G protein; GNAO 1; Go alpha; GO2-q chimeric G-protein; GTP-binding protein alpha o; GTP-binding protein alpha-o subunit; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha O polypeptide 1; Guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding regulatory protein 2; heterotrimeric guanine nucleotide-binding protein 1G; unnamed protein product
Gene Aliases: alphaO; AW050213; DEE17; EIEE17; G-ALPHA-o; Galphao; Gna0; GNAO; GNAO1; HG1G; HLA-DQB1; NEDIM; RATBPGTPC
UniProt ID: (Human) P09471, (Rat) P59215, (Mouse) P18872
Entrez Gene ID: (Human) 2775, (Rat) 50664, (Mouse) 14681
nucleotide binding
GTPase activity
obsolete small monomeric GTPase activity
protein binding
GTP binding
adenylate cyclase regulator activity
hydrolase activity
guanyl nucleotide binding
G-protein beta/gamma-subunit complex binding
G-protein coupled serotonin receptor binding
mu-type opioid receptor binding
metal ion binding
corticotropin-releasing hormone receptor 1 binding
signal transducer activity
GDP binding
protein complex binding
GTPase activating protein binding
heterotrimeric G-protein
vesicle docking involved in exocytosis
muscle contraction
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting serotonin receptor signaling pathway
dopamine receptor signaling pathway
locomotory behavior
regulation of heart contraction
negative regulation of insulin secretion
postsynaptic modulation of chemical synaptic transmission
aging
metabolic process
cellular process
response to organonitrogen compound
response to organic cyclic compound
forebrain development
neuron projection development
response to cytokine
response to drug
response to hydrogen peroxide
response to morphine
positive regulation of GTPase activity
negative regulation of calcium ion transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support