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          • Primary Antibodies ›
          • RUFY1 Antibodies

          Invitrogen

          RUFY1 Polyclonal Antibody

          View all (11) RUFY1 antibodies

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          Cite RUFY1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          RUFY1 Antibody in Immunocytochemistry (ICC/IF)
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          RUFY1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          RUFY1 Antibody (PA5-31399) in ICC/IF

          Immunocytochemistry analysis of RUFY1 in methanol-fixed MCF-7. Samples were incubated with RUFY1 polyclonal antibody (Product # PA5-31399) using a dilution of 1:200. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RUFY1 Antibody in Immunocytochemistry (ICC/IF)
          RUFY1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RUFY1 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-31399

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human RUFY1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 1% BSA, 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2548873

          Product Specific Information

          Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.

          Predicted reactivity: Mouse (95%), Rat (97%), Chicken (87%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: FYVE domain-containing protein; LABP1; FYVE-finger protein EIP1; la-binding protein 1; rab4-interacting protein; unnamed protein product; zinc finger FYVE domain-containing protein 12

          View more View less

          Gene Aliases: RABIP4; ZFYVE12

          View more View less

          UniProt ID: (Human) Q96T51

          View more View less

          Entrez Gene ID: (Human) 80230

          View more View less

          Function(s)
          protein binding SH3 domain binding protein binding, bridging SH2 domain binding identical protein binding metal ion binding
          Process(es)
          phosphatidylinositol biosynthetic process protein transport regulation of endocytosis endosomal vesicle fusion early endosome to Golgi transport
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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