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Invitrogen
PHOX2B Polyclonal Antibody
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FIGURE: 1 / 2
PHOX2B Antibody (PA5-35044) in ICC/IF
Immunocytochemistry analysis of PHOX2B in U251 cells. Samples were incubated with PHOX2B polyclonal antibody (Product # PA5-35044) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). PHOX2B immunoreactivity is localized to Nucleus si... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 104-130 amino acids from the central region of human PHOX2B
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
800 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with human and rat based on sequence homology.
Target Information
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dilated pupils 1; GENA 269; neuroblastoma paired-type homeobox protein; neuroblastoma Phox; paired like homeobox2B; paired mesoderm homeobox 2b; PHOX2B homeodomain protein; unnamed protein product
Gene Aliases: CCHS; Dilp1; NBLST2; NBPhox; PMX2B; Px2b
UniProt ID: (Human) Q99453, (Mouse) O35690
Entrez Gene ID: (Human) 8929, (Mouse) 18935
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
sequence-specific double-stranded DNA binding
protein binding
neuron migration
regulation of respiratory gaseous exchange by neurological system process
noradrenergic neuron differentiation
noradrenergic neuron development
brainstem development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
glial cell differentiation
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
cell differentiation in hindbrain
medullary reticular formation development
hindbrain tangential cell migration
positive regulation of transcription from RNA polymerase II promoter
autonomic nervous system development
enteric nervous system development
sympathetic nervous system development
parasympathetic nervous system development
neuron development
inner ear development
efferent axon development in a lateral line nerve
respiratory system development
retrotrapezoid nucleus neuron differentiation
sympathetic ganglion development
dopaminergic neuron differentiation
cellular response to BMP stimulus
positive regulation of cold-induced thermogenesis
neural crest cell migration involved in autonomic nervous system development
negative regulation of cell proliferation
neuron differentiation
skeletal muscle cell differentiation
regulation of respiratory gaseous exchange
negative regulation of neuron differentiation
positive regulation of neuron differentiation
cell development
neural nucleus development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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