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Invitrogen
ATG7 Polyclonal Antibody
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FIGURE: 1 / 8
ATG7 Antibody (PA5-35203) in ICC/IF
Immunocytochemistry analysis of ATG7 in U251 cells. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:200 for 2 hr at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:1,000 for 1hr. Cells were treated with Chloroquine (50 μM, 16hr), then fixed with 4% PFA (20 min), and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). ATG7 immunoreactivity is localized to autopha... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-35203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
800 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG7; APG7 autophagy 7-like; AT5G45900; ATAPG7; ATATG7; ATG12-activating enzyme E1 ATG7; AUTOPHAGY 7; AUTOPHAGY-RELATED 7; autophagy-related protein 7; DKFZp434N0735; hAGP7; K15I22.10; K15I22_10; PEROXISOME UNUSUAL POSITIONING 4; PEUP4; ubiquitin-activating enzyme E1-like protein; unnamed protein product
Gene Aliases: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; Atg7l; Gm21553; GSA7; SCAR31
UniProt ID: (Human) O95352, (Mouse) Q9D906
Entrez Gene ID: (Human) 10533, (Mouse) 74244
autophagosome assembly
mitophagy
mitophagy
liver development
protein lipidation
autophagy
cellular response to nitrogen starvation
mitochondrion organization
adult walking behavior
cell proliferation
cellular response to starvation
post-embryonic development
positive regulation of catabolic process
positive regulation of autophagy
negative regulation of cardiac muscle cell apoptotic process
macroautophagy
cerebellar Purkinje cell layer development
pyramidal neuron development
central nervous system neuron axonogenesis
cerebral cortex development
protein catabolic process
neuron projection development
regulation of protein ubiquitination
positive regulation of protein modification process
response to nutrient levels
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein modification by small protein conjugation
cellular response to stress
cellular response to reactive oxygen species
piecemeal microautophagy of nucleus
positive regulation of insulin secretion involved in cellular response to glucose stimulus
negative stranded viral RNA replication
response to starvation
regulation of circadian rhythm
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
negative regulation of neuron apoptotic process
innate immune response
positive regulation of protein catabolic process
negative regulation of phagocytosis
neurological system process
defense response to virus
cardiac muscle cell development
regulation of cell development
membrane organization
chaperone-mediated autophagy
protein lipidation involved in autophagosome assembly
negative regulation of programmed necrotic cell death
positive regulation of mucus secretion
cellular response to hyperoxia
amino acid homeostasis
negative regulation of sphingolipid biosynthetic process
cellular sphingolipid homeostasis
negative regulation of mitochondrial DNA replication
synaptic vesicle cycle
dendrite arborization
positive regulation of mitophagy
regulation of hemopoiesis
negative regulation of dendrite extension
cellular response to sodium arsenite
positive regulation of autophagosome assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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