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ATG7 Polyclonal Antibody

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3 Published Figures

5 References

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Additional Information:

ATG7 Antibody in Immunocytochemistry (ICC/IF)

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ATG7 Antibody (PA5-35203) in ICC/IF

Immunocytochemistry analysis of ATG7 in U251 cells. Samples were incubated with ATG7 polyclonal antibody (Product # PA5-35203) using a dilution of 1:200 for 2 hr at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:1,000 for 1hr. Cells were treated with Chloroquine (50 μM, 16hr), then fixed with 4% PFA (20 min), and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). ATG7 immunoreactivity is localized to autopha... View More

ATG7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATG7 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ATG7 Antibody in Western Blot, Immunohistochemistry, Immunohistochemistry (PFA fixed) (WB, IHC, IHC (PFA))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

ATG7 Antibody — FIGURE 3 Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. (A) DNA gel electrophoresis diagram. (B) The phosphorylation of H2AX in response to I/R-induced DNA damage ( *** P < 0.001 vs. control). Western blot analysis expression level of LC3 I/II ( * P < 0.05) (C) , Beclin1 ( *** P < 0.001), SQSTM1 ( * P < 0.05), and ATG7 ( ** P < 0.01) (D) and lysosomal associated proteins Lamp1 ( ** P < 0.01), Lamp2 ( * P < 0.05), and Cathepsin B ( * P < 0.05) (E) in total protein extract from RVA of I/R treated vs. control group. (F) The expression of NLRP3 ( *** P < 0.001), Caspase-1 ( ** P < 0.01), ASC ( ** P < 0.01), and IL-1beta ( ** P < 0.01). Results represent mean +- SE ( n = 3). The graph shows the densitometry quantification of western blot bands. Here control group represented Sham group. * P < 0.05, ** P < 0.01, and *** P < 0.001. {TM}

Immunogen image

Immunogen Image ATG7 Polyclonal Antibody

Product Details

PA5-35203

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

800 µg

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2552513

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Target Information

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: APG7; APG7-like; AT5G45900; ATAPG7; ATATG7; ATG12-activating enzyme E1 ATG7; AUTOPHAGY 7; AUTOPHAGY-RELATED 7; Autophagy-related protein 7; DKFZp434N0735; K15I22.10; K15I22_10; PEROXISOME UNUSUAL POSITIONING 4; PEUP4; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7

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Gene Aliases: 1810013K23Rik; Agp7; APG7L; ATG7; Atg7l; Gm21553

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UniProt ID: (Mouse) Q9D906

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Entrez Gene ID: (Mouse) 74244

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Function(s)

protein binding transcription factor binding small protein activating enzyme activity Atg12 activating enzyme activity Atg8 activating enzyme activity protein homodimerization activity ubiquitin-protein ligase

Process(es)

autophagosome assembly mitophagy liver development protein lipidation C-terminal protein lipidation cellular amino acid metabolic process transport autophagy cellular response to nitrogen starvation organelle organization mitochondrion organization adult walking behavior cellular response to starvation post-embryonic development positive regulation of autophagy protein transport macroautophagy positive regulation of macroautophagy cerebellar Purkinje cell layer development pyramidal neuron development central nervous system neuron axonogenesis cerebral cortex development protein catabolic process neuron projection development regulation of protein ubiquitination positive regulation of protein modification process protein modification by small protein conjugation piecemeal microautophagy of nucleus suppression by virus of host autophagy negative stranded viral RNA replication response to starvation positive regulation of apoptotic process negative regulation of apoptotic process late nucleophagy positive regulation of protein catabolic process neurological system process defense response to virus cardiac muscle cell development regulation of cell development negative regulation of cell death membrane organization positive regulation of mucus secretion cellular response to hyperoxia regulation of mitophagy regulation of hemopoiesis negative regulation of histone H4-K16 acetylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG7 Polyclonal Antibody

ATG7 Antibody (PA5-35203) in ICC/IF

Product Details

PA5-35203

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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