Search
Invitrogen
Phospho-Cardiac Troponin I (Ser150) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
Phospho-Cardiac Troponin I (Ser150) Antibody (PA5-35410) in WB
Western blot of Cardiac Troponin I in mouse heart lysate showing specific immunolabeling of a band at ~25 kDa corresponding to Phospho-Cardiac Troponin I (Ser150) polyclonal antibody (Product # PA5-35410) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase (1,200 units for 30 minutes).
Product Details
PA5-35410
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse TNNI3 conjugated to KLH
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/mL BSA
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with human and non-human primate based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Target Information
Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); MGC116817; sarcomeric protein; TNNI3; Tpn1; troponin 1, type 3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; unnamed protein product
Gene Aliases: CMD1FF; CMD2A; CMH7; Ctni; RCM1; Tn1; TnI; TNNC1; TNNI3
UniProt ID: (Human) P19429, (Rat) P23693, (Mouse) P48787
Entrez Gene ID: (Human) 7137, (Rat) 29248, (Mouse) 21954
vasculogenesis
regulation of systemic arterial blood pressure by ischemic conditions
skeletal muscle contraction
cellular calcium ion homeostasis
regulation of muscle contraction
regulation of smooth muscle contraction
striated muscle contraction
heart development
regulation of cardiac muscle contraction by calcium ion signaling
muscle filament sliding
negative regulation of ATPase activity
ventricular cardiac muscle tissue morphogenesis
heart contraction
cardiac muscle contraction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support