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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody

          Advanced Verification
          1 Reference
          View all (88) ERK1/2 antibodies

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          Cite Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          • Advanced Verification (1)
          Phospho-ERK1/ERK2 (Tyr204) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-ERK1/ERK2 (Tyr204) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-ERK1/ERK2 (Tyr204) Antibody (PA5-36776) in ICC/IF

          Immunofluorescence analysis of Phospho-ERK1/ERK2 (Tyr204) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 min, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ERK1/ERK2 (Tyr204) Rabbit Polyclonal Antibody ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Phospho-ERK1/ERK2 (Tyr204) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-ERK1/ERK2 (Tyr204) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-ERK1/ERK2 (Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Tyr204) Antibody in Western Blot (WB)
          Phospho-ERK1/ERK2 (Tyr204) Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody

          Product Details

          PA5-36776

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2553723

          Product Specific Information

          This antibody detects endogenous protein at a molecular weight of 42 and 44 kDa.

          Purity is >95% by SDS-PAGE.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ERK-2; erk1 erk2; Erk1; cancer susceptibility protein; ERK1; MAP kinase; mitogen-activated protein kinase; ERK1b; ERK1c; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase 1; extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAPK 1; MAPK 2; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; pp42/MAP kinase; protein serine/threonine kinase ERK1b; protein tyrosine kinase ERK2; unnamed protein product

          View more View less

          Gene Aliases: 9030612K14Rik; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK2; MNK1; Mtap2k; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

          View more View less

          UniProt ID: (Human) P28482, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708

          View more View less

          Entrez Gene ID: (Human) 5594, (Human) 5595, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689

          View more View less

          Function(s)
          phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding double-stranded DNA binding protein kinase binding mitogen-activated protein kinase kinase kinase binding scaffold protein binding
          Process(es)
          autophagosome assembly MAPK cascade negative regulation of T cell mediated immune response to tumor cell protein phosphorylation cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway insulin receptor signaling pathway response to toxic substance organ morphogenesis negative regulation of autophagy positive regulation of autophagy positive regulation of macrophage chemotaxis neural crest cell development Schwann cell development phosphorylation peptidyl-serine phosphorylation sensory perception of pain regulation of ossification BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels positive regulation of telomere maintenance negative regulation of proteasomal ubiquitin-dependent protein catabolic process response to lipopolysaccharide regulation of stress-activated MAPK cascade cellular response to amino acid starvation intracellular signal transduction peptidyl-tyrosine autophosphorylation ERBB2-ERBB3 signaling pathway TORC1 signaling outer ear morphogenesis myelination signal transduction in response to DNA damage response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter decidualization insulin-like growth factor receptor signaling pathway thymus development modulation of synaptic transmission protein stabilization negative regulation of T cell activation cartilage development stress-activated MAPK cascade regulation of intracellular pH regulation of cytoskeleton organization Bergmann glial cell differentiation face development lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway protein-containing complex assembly ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance negative regulation of cholesterol efflux cellular response to toxic substance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response negative regulation of TORC1 signaling positive regulation of xenophagy regulation of early endosome to late endosome transport arachidonic acid metabolic process regulation of transcription from RNA polymerase II promoter apoptotic process chemotaxis chemical synaptic transmission learning or memory positive regulation of peptidyl-threonine phosphorylation response to nicotine ERBB signaling pathway positive regulation of skeletal muscle tissue regeneration positive regulation of cholesterol biosynthetic process positive regulation of smoothened signaling pathway chemokine-mediated signaling pathway heart development positive regulation of cell proliferation diadenosine tetraphosphate biosynthetic process cytosine metabolic process positive regulation of cell migration response to estradiol response to testosterone mammary gland epithelial cell proliferation cellular response to platelet-derived growth factor stimulus response to cocaine positive regulation of protein import into nucleus response to hydrogen peroxide response to estrogen estrous cycle negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of cardiac muscle cell proliferation long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to methionine cellular response to cAMP cellular response to epidermal growth factor stimulus cellular response to peptide hormone stimulus cellular response to prostaglandin E stimulus cellular response to lipid response to alcohol cellular response to insulin-like growth factor stimulus placenta development
          It has to be done as per old AB suggested Products section.

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