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Invitrogen
Phospho-GAB1 (Tyr627) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-GAB1 (Tyr627) Antibody (PA5-36846) in WB
Western blot was performed using Anti-Phospho-GAB1 (Tyr627) Polyclonal Antibody (Product # PA5-36846) and a 110 kDa band corresponding to Phospho-GAB1 (Tyr627) was enhanced upon treatment with EGF, Insulin, NGF and Heregulin across cell lines tested. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 treated with EGF (200 ng/mL for 10 mins) (Lane 2), 3T3-L1 treated with Insulin (100 nm for 10 min... View More
Product Details
PA5-36846
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 627
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 76 and 110 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: deafness, autosomal recessive 26; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; OTTHUMP00000220263; symbol withdrawn, see GAB1; unnamed protein product
Gene Aliases: AA408973; AW107238; DFNB26; GAB1
UniProt ID: (Human) Q13480, (Mouse) Q9QYY0
Entrez Gene ID: (Human) 2549, (Mouse) 14388
angiogenesis
signal transduction
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
intracellular signal transduction
endothelial cell chemotaxis
vascular endothelial growth factor signaling pathway
vasodilation
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of protein kinase B signaling
cellular response to mechanical stimulus
activation of MAPK activity
response to oxidative stress
activation of JUN kinase activity
heart development
epidermis development
regulation of cell migration
positive regulation of MAPK cascade
platelet-derived growth factor receptor signaling pathway
labyrinthine layer development
interleukin-6-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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