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Invitrogen
Phospho-PAK4/6/7 (Ser474) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-PAK4/6/7 (Ser474) Antibody (PA5-36865) in WB
Western blot analysis of Phospho-PAK4+PAK5+PAK6 pSer474 using Phospho-PAK4+PAK5+PAK6 pSer474 polyclonal antibody (Product # PA5-36865) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (E0.1 ng/mL, 30 min), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).
Product Details
PA5-36865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 72 kDa (PAK4), 82 kDa (PAK6) and 90 (PAK7).
Purity is >95% by SDS-PAGE.
Target Information
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p21 (RAC1) activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A)-activated kinase 4; p21(CDKN1A)-activated kinase 6; p21(CDKN1A)-activated kinase 7; p21-activated kinase 4; p21-activated kinase 5; p21-activated kinase 6; p21-activated kinase 7; p21CDKN1A-activated kinase 7; PAK 4/5/6; PAK 4/6/7; PAK-4; PAK-5; PAK-6; PAK-7; PAK1-related kinase; PAK4/5/6; protein kinase PAK5; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; Serine/threonine-protein kinase PAK 5; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 7; serine/threonine-protein kinase PAK7; unnamed protein product
Gene Aliases: KIAA1142; KIAA1264; PAK4; PAK5; PAK6; PAK7
UniProt ID: (Human) O96013, (Human) Q9P286, (Human) Q9NQU5
Entrez Gene ID: (Human) 10298, (Human) 57144, (Human) 56924
regulation of transcription, DNA-templated
apoptotic process
cytoskeleton organization
learning
memory
locomotory behavior
cellular response to starvation
intracellular signal transduction
regulation of MAPK cascade
neuron projection arborization
neuron projection extension
regulation of cell growth
signal transduction
cell migration
positive regulation of angiogenesis
protein stabilization
dendritic spine development
cell-cell adhesion
positive regulation of focal adhesion disassembly
negative regulation of endothelial cell apoptotic process
cell proliferation
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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