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Invitrogen
Phospho-ATF2 (Ser44, Ser62) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-37531
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around phosphorylation site of serine 62 or 44 (N-D-S(p)-V-I) derived from Human ATF2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control for Western blot is MDA231 cells; suggested positive control for IHC is human breast carcinoma.
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating 2; ATF2; bZIP transcription factor; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP response element-binding protein (HB16); cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2; MGC111558; mXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354; RATF2; unnamed protein product
Gene Aliases: Atf-2; CRE-BP; CRE-BP1; CREB-2; CREB2; D130078H02Rik; HB16; mXBP; Tg(Gzma-Klra1)7Wum; TREB7
UniProt ID: (Human) P15336, (Mouse) P16951, (Rat) Q00969
Entrez Gene ID: (Human) 1386, (Mouse) 11909, (Rat) 81647
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
H4 histone acetyltransferase activity
protein kinase binding
cAMP response element binding
identical protein binding
protein homodimerization activity
leucine zipper domain binding
sequence-specific DNA binding
histone H2B acetyltransferase activity
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
cellular glucose homeostasis
in utero embryonic development
NK T cell differentiation
liver development
positive regulation of protein phosphorylation
hematopoietic progenitor cell differentiation
outflow tract morphogenesis
brainstem development
growth plate cartilage chondrocyte differentiation
growth plate cartilage chondrocyte proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
lipid metabolic process
apoptotic process
response to osmotic stress
cellular response to DNA damage stimulus
vacuole organization
JNK cascade
heart development
gene expression
positive regulation of gene expression
negative regulation of angiogenesis
abducens nucleus development
hypoglossal nucleus development
facial nucleus development
BMP signaling pathway
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
cellular response to oxidative stress
p38MAPK cascade
mRNA transcription from RNA polymerase II promoter
positive regulation of neuron apoptotic process
fat cell differentiation
positive regulation of gene expression, epigenetic
positive regulation of transcription from RNA polymerase II promoter
negative regulation of epithelial cell proliferation
white fat cell differentiation
neurofilament cytoskeleton organization
detection of cell density
adipose tissue development
cellular response to anisomycin
motor neuron apoptotic process
amelogenesis
hepatocyte apoptotic process
cellular response to virus
positive regulation of cardiac muscle myoblast proliferation
positive regulation of mitochondrial membrane permeability involved in apoptotic process
intrinsic apoptotic signaling pathway in response to hypoxia
cellular response to leucine starvation
peptidyl-threonine phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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