Search

  • Fale Conosco
  • Pedido rápido

Invitrogen

Phospho-HSF1 (Thr142) Polyclonal Antibody

View all (59) HSF1 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation'] || 'Additional Information'}}: {{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:

{{$ctrl.translations[$ctrl.isSkuNotesCollapsed ? 'antibody.pdp.skuNotes.viewMore' : 'antibody.pdp.skuNotes.viewLess']}}
Phospho-HSF1 (Thr142) Polyclonal Antibody

Product Details

PA5-37600

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554208

Product Specific Information

A suggested positive control for IHC is human brain tissue.

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: HSF1; unnamed protein product

View more View less

Gene Aliases: Hsf1alpha; Hsf1beta; HSTF; HSTF 1; HSTF1

View more View less

UniProt ID: (Human) Q00613, (Mouse) P38532

View more View less

Entrez Gene ID: (Human) 3297, (Mouse) 15499

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding heat shock protein binding chromatin DNA binding identical protein binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding translation elongation factor binding general transcription initiation factor binding sequence-specific double-stranded DNA binding STAT family protein binding sequence-specific single stranded DNA binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade in utero embryonic development embryonic placenta development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation defense response female meiotic division spermatogenesis negative regulation of cell proliferation response to heat gene expression positive regulation of gene expression negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process negative regulation of protein complex assembly positive regulation of protein modification process response to estradiol response to lipopolysaccharide negative regulation of tumor necrosis factor production response to testosterone cellular response to heat cellular response to unfolded protein positive regulation of multicellular organism growth protein kinase B signaling positive regulation of macrophage differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter embryonic process involved in female pregnancy positive regulation of stress granule assembly protein-containing complex assembly cellular response to hydrogen peroxide cellular response to amino acid stimulus cellular response to cadmium ion cellular response to copper ion cellular response to estradiol stimulus cellular response to radiation cellular response to gamma radiation cellular response to diamide negative regulation of inclusion body assembly positive regulation of inclusion body assembly positive regulation of cold-induced thermogenesis regulation of cellular response to heat positive regulation of apoptotic DNA fragmentation cellular response to sodium arsenite cellular response to angiotensin negative regulation of double-strand break repair via nonhomologous end joining positive regulation of apoptotic signaling pathway
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support