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Invitrogen
Phospho-SF1 (Ser82) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-37765
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control for Western blot is HUVEC cells; suggested positive control for IHC is human brain tissue.
Target Information
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: branch point-binding protein; CW17; CW17R; M ZFM1; mammalian branch point-binding protein; SF; transcription factor ZFM1; unnamed protein product; zinc finger gene in MEN1 locus; zinc finger protein 162
Gene Aliases: BBP; D11S636; MBBP; MZFM; WBP4; ZCCHC25; ZFM1; Zfp162; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536, (Mouse) 22668
mRNA splicing, via spliceosome
male sex determination
nuclear body organization
Leydig cell differentiation
mRNA cis splicing, via spliceosome
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
spliceosomal complex assembly
mRNA 3'-splice site recognition
negative regulation of transcription, DNA-templated
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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