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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody

          View all (59) AKT Pan antibodies

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          Cite Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody (PA5-37814) in ICC/IF

          Immunocytochemical analysis of Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) in methanol-fixed Hela cells, using Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody (Product # PA5-37814). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Western Blot (WB)
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Western Blot (WB)
          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody

          Product Details

          PA5-37814

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:100
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2554422

          Product Specific Information

          A suggested positive control for Western blot is HepG2 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; Akt2; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB alpha; PKB beta; PKB gamma; PKBgamma/Akt3; PKBgamma1; protein kinase Akt-2; protein kinase Akt-3; protein kinase B alpha; protein kinase B beta; protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; proto-oncogene c-AKT; proto-oncogene product; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-gamma serine/threonine protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; similar to rat serine-threonine protein kinase; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; tumor rejection antigen peptide pRLib; unnamed protein product; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: 2410016A19Rik; AKT; D930002M15Rik; HIHGHH; LTR-akt; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBB; PKBBETA; PKBG; PRKBA; PRKBB; PRKBG; RAC; RAC-ALPHA; RAC-BETA; RAC-gamma; RAC-PK-gamma; STK-2

          View more View less

          UniProt ID: (Human) P31749, (Human) P31751, (Human) Q9Y243, (Rat) P47196, (Mouse) P31750, (Mouse) Q60823, (Rat) P47197, (Mouse) Q9WUA6, (Rat) Q63484

          View more View less

          Entrez Gene ID: (Human) 207, (Human) 208, (Human) 10000, (Rat) 24185, (Mouse) 11651, (Mouse) 11652, (Rat) 25233, (Mouse) 23797, (Rat) 29414

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding molecular function activator activity protein kinase C binding GTPase activating protein binding protein phosphatase 2A binding protein serine/threonine kinase binding
          Process(es)
          positive regulation of protein phosphorylation glucose metabolic process protein phosphorylation insulin receptor signaling pathway positive regulation of signal transduction positive regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of sodium ion transport positive regulation of glucose metabolic process positive regulation of cell migration positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance response to insulin cellular response to insulin stimulus negative regulation of RNA splicing intracellular signal transduction negative regulation of apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein stabilization positive regulation of positive chemotaxis cellular response to high light intensity protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity retinal rod cell apoptotic process positive regulation of cap-dependent translational initiation negative regulation of PERK-mediated unfolded protein response positive regulation of cell motility osteoblast differentiation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway regulation of glycogen biosynthetic process nitric oxide biosynthetic process activation-induced cell death of T cells response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell proliferation response to heat response to hormone negative regulation of autophagy positive regulation of endothelial cell migration negative regulation of gene expression fibroblast migration regulation of neuron projection development negative regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of cell growth regulation of cell migration T cell costimulation negative regulation of protein ubiquitination TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of peptidyl-serine phosphorylation cellular response to stress response to fluid shear stress interleukin-18-mediated signaling pathway positive regulation of protein import into nucleus regulation of apoptotic process anoikis regulation of mRNA stability positive regulation of fat cell differentiation negative regulation of Notch signaling pathway negative regulation of proteolysis nitric oxide metabolic process positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation positive regulation of protein metabolic process positive regulation of protein kinase B signaling excitatory postsynaptic potential response to growth hormone mammary gland epithelial cell differentiation response to UV-A protein localization to mitochondrion response to growth factor cellular response to epidermal growth factor stimulus maintenance of protein location in mitochondrion cellular response to rapamycin negative regulation of release of cytochrome c from mitochondria vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway maternal placenta development response to ischemia glycogen metabolic process glycogen biosynthetic process translation inflammatory response cellular response to DNA damage stimulus smoothened signaling pathway germ cell development apoptotic mitochondrial changes gene expression positive regulation of fibroblast migration positive regulation of mitochondrial membrane potential response to muscle activity protein ubiquitination spinal cord development lateral motor column neuron differentiation oogenesis stage cell projection organization protein catabolic process regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels response to food regulation of protein localization negative regulation of superoxide anion generation cellular response to heat cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis positive regulation of apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response cellular response to sorbitol cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis negative regulation of calcium import into the mitochondrion cellular response to peptide regulation of aerobic respiration regulation of mitochondrion organization positive regulation of angiogenesis positive regulation of cell migration involved in sprouting angiogenesis positive regulation of vascular endothelial cell proliferation positive regulation of artery morphogenesis negative regulation of cellular senescence protein modification process fat cell differentiation regulation of cell cycle cytoplasmic translation gluconeogenesis translational initiation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport cytoskeleton organization cellular response to starvation phosphorylation cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation protein K29-linked ubiquitination SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling Golgi to plasma membrane protein transport proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process positive regulation of cilium assembly negative regulation of translational initiation positive regulation of translational initiation protein maturation regulation of vesicle-mediated transport protein localization to cilium protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion tricarboxylic acid metabolic process protein K6-linked ubiquitination tRNA (guanine-N7)-methylation cGAS/STING signaling pathway negative regulation of TORC1 signaling positive regulation of TOR signaling positive regulation of cell size brain morphogenesis homeostasis of number of cells within a tissue response to hypoxia cellular response to hormone stimulus positive regulation of adipose tissue development
          It has to be done as per old AB suggested Products section.

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