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Invitrogen
Phospho-MST1/MST2 (Thr183) Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-40255
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from human Krs-1/2 around the phosphorylation site of T183.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-2; MST1/C STK4; protein kinase; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine-protein kinase Krs-1; serine/threonine-protein kinase Krs-2; Sps1/Ste20-related kinase 3; STE20-like kinase MST1; STE20-like kinase MST2; sterile 20-like kinase 1; unnamed protein product
Gene Aliases: 0610042I06Rik; Kas-2; KRS1; KRS2; mess1; MST; MST1; MST2; Mst3; Ste20; YSK3
UniProt ID: (Human) Q13188, (Human) Q13043, (Mouse) Q9JI10, (Mouse) Q9JI11
Entrez Gene ID: (Human) 6788, (Human) 6789, (Mouse) 56274, (Mouse) 58231
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
identical protein binding
protein serine/threonine kinase activator activity
protein serine kinase activity
transcription regulator activator activity
protein homodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
cell morphogenesis
positive regulation of protein phosphorylation
protein phosphorylation
protein import into nucleus
apoptotic process
signal transduction
anatomical structure morphogenesis
peptidyl-serine phosphorylation
positive regulation of peptidyl-serine phosphorylation
hippo signaling
positive regulation of hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
regulation of MAPK cascade
protein autophosphorylation
protein stabilization
protein tetramerization
negative regulation of canonical Wnt signaling pathway
positive regulation of substrate-dependent cell migration, cell attachment to substrate
regulation of gene expression
negative regulation of growth
negative regulation of cell growth involved in contact inhibition
protein localization to centrosome
neural tube formation
endocardium development
central nervous system development
negative regulation of cell proliferation
positive regulation of fat cell differentiation
positive regulation of JNK cascade
negative regulation of organ growth
centrosome separation
positive regulation of protein kinase B signaling
primitive hemopoiesis
cell differentiation involved in embryonic placenta development
regulation of cell differentiation involved in embryonic placenta development
hepatocyte apoptotic process
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
patterning of blood vessels
keratinocyte differentiation
positive regulation of vascular associated smooth muscle cell apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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