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Invitrogen

Phospho-MST1/MST2 (Thr183) Polyclonal Antibody

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Phospho-MST1/MST2 (Thr183) Polyclonal Antibody

Product Details

PA5-40255

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

1:100-1:300
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized peptide derived from human Krs-1/2 around the phosphorylation site of T183.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.5% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607978

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1/C STK4; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2; sterile 20-like kinase 1; unnamed protein product; Yeast Sps1/Ste20-related kinase 3

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Gene Aliases: 0610042I06Rik; AI447339; AU020804; Kas-2; KRS1; KRS2; Mess1; MST; MST1; MST2; Mst3; Ste20; STK3; STK4; YSK3

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UniProt ID: (Mouse) Q9JI10, (Mouse) Q9JI11

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Entrez Gene ID: (Human) 6788, (Human) 6789, (Mouse) 56274, (Mouse) 58231

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding identical protein binding protein serine/threonine kinase activator activity protein serine kinase activity transcription regulator activator activity protein homodimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding nucleotide binding MAP kinase kinase kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding transcription factor binding protein dimerization activity non-receptor serine/threonine protein kinase
Process(es)
protein phosphorylation protein import into nucleus apoptotic process signal transduction regulation of gene expression hippo signaling positive regulation of hippo signaling intracellular signal transduction positive regulation of apoptotic process regulation of MAPK cascade negative regulation of growth protein stabilization protein tetramerization negative regulation of cell growth involved in contact inhibition protein localization to centrosome negative regulation of canonical Wnt signaling pathway cell morphogenesis positive regulation of protein phosphorylation anatomical structure morphogenesis peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation protein autophosphorylation positive regulation of substrate-dependent cell migration, cell attachment to substrate MAPK cascade activation of MAPKK activity neural tube formation endocardium development regulation of mitotic cell cycle central nervous system development negative regulation of cell proliferation phosphorylation signal transduction by protein phosphorylation stress-activated protein kinase signaling cascade positive regulation of protein binding positive regulation of fat cell differentiation positive regulation of JNK cascade negative regulation of organ growth positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling primitive hemopoiesis cell differentiation involved in embryonic placenta development regulation of cell differentiation involved in embryonic placenta development hepatocyte apoptotic process positive regulation of extrinsic apoptotic signaling pathway via death domain receptors patterning of blood vessels keratinocyte differentiation
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