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Invitrogen
Galectin 9 Polyclonal Antibody
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FIGURE: 1 / 3
Galectin 9 Antibody (PA5-47503) in ICC/IF
Immunocytochemistry analysis of Galectin 9 in immersion fixed mouse splenocytes. Samples were incubated in Galectin 9 polyclonal antibody (Product # PA5-47503) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-47503
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse Galectin-9 Ala2-Thr353 (aa 148-178 deletion)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Gal9; Galectin9; HOM HD 21; lectin, galactose binding, soluble 5; unnamed protein product
Gene Aliases: gal-9; galectin-9; LGALS35; Lgals5
UniProt ID: (Mouse) O08573
Entrez Gene ID: (Mouse) 16859
positive regulation of cytokine production
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
transforming growth factor beta receptor signaling pathway
female pregnancy
positive regulation of gene expression
negative regulation of gene expression
regulation of T cell chemotaxis
response to lipopolysaccharide
negative regulation of interferon-gamma production
positive regulation of chemokine production
positive regulation of interleukin-1 production
positive regulation of interleukin-10 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
negative regulation of natural killer cell activation
regulation of natural killer cell differentiation
regulation of T cell differentiation in thymus
positive regulation of macrophage activation
receptor clustering
negative regulation of natural killer cell degranulation
positive regulation of innate immune response
maintenance of protein location
positive regulation of regulatory T cell differentiation
negative regulation of inflammatory response
positive regulation of oxidoreductase activity
positive regulation of SMAD protein import into nucleus
cellular response to virus
positive regulation of defense response to bacterium
regulation of T-helper 17 type immune response
positive regulation of T cell migration
negative regulation of CD4-positive, alpha-beta T cell proliferation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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