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Invitrogen
uPAR Polyclonal Antibody
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FIGURE: 1 / 2
uPAR Antibody (PA5-47724) in IHC (F)
Immunohistochemical analysis of uPAR in perfusion fixed frozen sections of mouse kidney. Samples were incubated in uPAR polyclonal antibody (Product # PA5-47724) using a dilution of 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells.
Product Details
PA5-47724
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1 Leu24-Thr297
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human uPAR is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The urokinase-type plasminogen activator receptor is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes. The human PLAUR cDNA encodes 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. It produces a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. PLAUR, also known as UPAR, is directly associated with the carbohydrate-binding domain of SELL in the membrane of neutrophils, an association analogous to that between PLAUR and beta-2 integrins. PLAUR-mediated calcium mobilization is SELL dependent. UPAR mRNA levels correlate with the invasive potential of endometrial carcinomas and show a 33-fold increase in UPAR mRNA levels in advanced clinical stage endometrial tumors compared with normal endometrial tissue. Furthermore, the increase in UPAR mRNA levels correlated linearly with the progression of disease stage. UPAR protein expressioin correlated positively with rate of recurrence and mortality in patients with endometrial cancer. UPAR appears to be a useful prognostic marker for advanced endometrial cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD87; sCD87; soluble CD87; U-PAR; urokinase plasminogen activator receptor; Urokinase plasminogen activator surface receptor; urokinase-type plasminogen activator receptor
Gene Aliases: Cd87; Plaur; u-PAR; uPAR
UniProt ID: (Mouse) P35456
Entrez Gene ID: (Mouse) 18793
positive regulation of protein phosphorylation
positive regulation of gene expression
urokinase plasminogen activator signaling pathway
negative regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epidermal growth factor receptor signaling pathway
mesenchymal cell differentiation
epithelial cell differentiation involved in prostate gland development
positive regulation of release of cytochrome c from mitochondria
negative regulation of intrinsic apoptotic signaling pathway
negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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