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Invitrogen
Angiopoietin 3 Polyclonal Antibody
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FIGURE: 1 / 1
Angiopoietin 3 Antibody (PA5-47921) in IHC (F)
Immunohistochemical analysis of Angiopoietin 3 in perfusion fixed frozen sections of rat kidney. Samples were incubated in Angiopoietin 3 polyclonal antibody (Product # PA5-47921) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-47921
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse Angiopoietin-3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2 or rhAngoipoietin-3 was observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. Ang-1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), is a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression is restricted to sites of vascular remodeling. Ang-3 and Ang-4 represent the mouse and human counterparts of the same gene locus. The structural divergence of Ang-3 and Ang-4 cause their divergent functions. Ang-3 and Ang-4 have very different distributions in their respective species, and Ang-3 appears to act as an antagonist while Ang-4 appears to function as an agonist. Ang-3 and Ang-4 share all the main structural characteristics of Ang-1 and Ang-2 and are homologous throughout the signal peptide, N-terminal region, coiled-coil segment and Fibrinogen-like domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGP4; angiopoietin-3; Angiopoietin3; Angiopoietin4; unnamed protein product
Gene Aliases: Agpt4; ANG-3; ANG-4; Ang3
UniProt ID: (Mouse) Q9WVH6
Entrez Gene ID: (Mouse) 11602
angiogenesis
signal transduction
Notch signaling pathway
endoderm development
blood coagulation
positive regulation of endothelial cell migration
negative regulation of angiogenesis
negative regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
negative regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
Tie signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
cellular response to hypoxia
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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