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          • Primary Antibodies ›
          • NDUFA4 Antibodies

          Invitrogen

          NDUFA4 Polyclonal Antibody

          1 Published Figure
          2 References
          View all (5) NDUFA4 antibodies

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          Cite NDUFA4 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (1)
          NDUFA4 Antibody in Immunocytochemistry (ICC/IF)
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          NDUFA4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          NDUFA4 Antibody (PA5-51021) in ICC/IF

          Immunofluorescence analysis of NDUFA4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NDUFA4 Rabbit Polyclonal Antibody (Product # PA5-51021) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          NDUFA4 Antibody in Immunocytochemistry (ICC/IF)
          NDUFA4 Antibody in Western Blot (WB)
          NDUFA4 Antibody in Western Blot (WB)
          NDUFA4 Antibody in Western Blot (WB)
          NDUFA4 Polyclonal Antibody

          Product Details

          PA5-51021

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          1:100-1:300
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          ELISA (ELISA)

          1:10,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthesized peptide derived from the C-terminal region of human NDUFA4.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol, 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2636469

          Product Specific Information

          NDUFA4 Polyclonal Antibody detects endogenous levels of NDUFA4 protein.

          Target Information

          Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. {ECO:0000269; PubMed:22902835}.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CI-MLRQ; Complex I 9kDa subunit; Complex I-MLRQ; Cytochrome c oxidase subunit FA4; Cytochrome c oxidase subunit NDUFA4; EC 1.6.5.3; EC 1.6.99.3; mitochondrial respiratory chain associated factor 1; mitochondrial stress response 1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4 (9 kDa, MLRQ); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4; NADH-ubiquinone oxidoreductase MLRQ subunit; NDUFA4

          View more View less

          Gene Aliases: CI-9k; CI-MLRQ; COXFA4; MC4DN21; MISTR1; MLRQ; MRCAF1; NDUFA4

          View more View less

          UniProt ID: (Human) O00483, (Mouse) Q62425

          View more View less

          Entrez Gene ID: (Human) 4697, (Rat) 681024, (Mouse) 17992

          View more View less

          Function(s)
          protein binding NADH dehydrogenase (ubiquinone) activity macromolecular complex binding cytochrome-c oxidase activity protein complex binding oxidoreductase
          Process(es)
          mitochondrial electron transport, NADH to ubiquinone mitochondrial electron transport, cytochrome c to oxygen cellular respiration hydrogen ion transmembrane transport transport oxidation-reduction process
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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