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Invitrogen
JMJD3 Polyclonal Antibody
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FIGURE: 1 / 11
JMJD3 Antibody (PA5-72751) in ICC/IF
Immunocytochemistry of K562 cells using JMJD3 Polyclonal Antibody (Product # PA5-72751) at 2.5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-72751
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
JMJD3 antibody for a 16 amino acid synthetic peptide from near the amino terminus of human JMJD3. The immunogen is within amino acids 1370 - 1420 of JMJD3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(27) demethylase 6B; jmjC domain-containing protein 3; jumonji domain containing 3, histone lysine demethylase; jumonji domain-containing 3; jumonji domain-containing protein 3; KIAA0346; lysine (K)-specific demethylase 6B
Gene Aliases: JMJD3; NEDCFSA; NEDSST
UniProt ID: (Human) O15054
Entrez Gene ID: (Human) 23135, (Rat) 363630
inflammatory response to antigenic stimulus
chromatin remodeling
heart development
regulation of gene expression
response to activity
hippocampus development
cell fate commitment
endothelial cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mesodermal cell differentiation
cardiac muscle cell differentiation
response to fungicide
cellular response to hydrogen peroxide
positive regulation of cold-induced thermogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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