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Invitrogen
Annexin V Polyclonal Antibody
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FIGURE: 1 / 3
Annexin V Antibody (PA5-78784) in WB
Western blot was performed using anti-Annexin V Polyclonal Antibody (Product # PA5-78784) and a 35 kDa band corresponding to Annexin V was observed across the cell lines and tissue tested. Whole cell extracts (30 µg) of HeLa (Lane 1), Jurkat (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), Hep G2 (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78784
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Annexin V recombinant protein (Position: A2-D320).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg/mL trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Lung Tissue, Rat Brain Tissue, Rat Cardiac Muscle Tissue, Rat Kidney Tissue, HELA whole cell, SMMC whole cell, A549 whole cell, SGC whole cell. IHC: Human Intestinal Cancer tissue, Human Mammary Cancer tissue, Mouse Kidney tissue, Rat Kidney tissue IHC-F: rat kidney tissue, human placenta tissue. ICC/IF: U20S cell, RH35 cell. Flow: PC-3 cell, SiHa cell.
Target Information
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anchorin CII; Annexin V; annexin-5; anticoagulant precursor (5' end put.); putative; ANXA5; calphobindin I; CBP-I; endonexin II; epididymis secretory protein Li 7; lipocortin V; lipocortin-V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; thromboplastin inhibitor; unnamed protein product; VAC-alpha; vascular anticoagulant-alpha
Gene Aliases: ANX5; CPB-I; ENX2; HEL-S-7; LC5; PP4; RPRGL3; VAC-alph
UniProt ID: (Human) P08758, (Rat) P14668, (Mouse) P48036
Entrez Gene ID: (Human) 308, (Rat) 25673, (Mouse) 11747
signal transduction
negative regulation of apoptotic process
negative regulation of coagulation
negative regulation of blood coagulation
positive regulation of apoptotic process
response to calcium ion
calcium ion transmembrane transport
cellular response to lead ion
response to thyroid hormone
cellular response to gonadotropin-releasing hormone
regulation of sperm motility
negative regulation of prolactin secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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