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Invitrogen
TCP-1 delta Polyclonal Antibody
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FIGURE: 1 / 14
TCP-1 delta Antibody (PA5-78954) in ICC/IF
Immunocytochemistry analysis of CCT4 using anti-CCT4 antibody (Product # PA5-78954) . CCT4 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CCT4 antibody (Product # PA5-78954) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78954
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human CCT4 recombinant protein (Position: R452-R539).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U20S whole cell, human SW620 whole cell, human MCF-7 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue. IHC: mouse kidney tissue, rat kidney tissue, human mammary cancer tissue IHC-F: mouse brain tissue, rat brain tissue. ICC/IF: U20S cell, LOVO Cell, Neuro-2a Cell, NRK Cell, SHG-44 Cell. Flow: PC-3 cell, U251 cell, K562 cell.
Target Information
The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCT containing TCP-1-delta; CCT-delta; chaperonin containing T-complex polypeptide 1 subunit 4; chaperonin containing t-complex polypeptide 1, delta subunit; chaperonin containing TCP1, subunit 4 (delta); chaperonin subunit 4 (delta); cytosolic chaperonin-containing t-complex peptide-1; stimulator of TAR RNA-binding; T complex protein 1, delta; TCP-1-delta; unnamed protein product
Gene Aliases: 2610204B21Rik; A45; CCT-DELTA; Cctd; SRB
UniProt ID: (Human) P50991, (Rat) Q7TPB1, (Mouse) P80315
Entrez Gene ID: (Human) 10575, (Rat) 29374, (Mouse) 12464
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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