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Invitrogen
CD80 (B7-1) Polyclonal Antibody
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FIGURE: 1 / 7
CD80 (B7-1) Antibody (PA5-79001) in IHC (P)
Immunohistochemistry (Paraffin) analysis of CD80 (B7-1) in paraffin-embedded section of human tonsil tissue using CD80 (B7-1) Polyclonal Antibody (Product # PA5-79001). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution over... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human CD80 recombinant protein (Position: V35-L244).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Daudi whole cell, human Raji whole cell, human Jurkat whole cell, rat spleen tissue, mouse RAW2647 whole cell, mouse ANA-1 whole cell. IHC: human tonsil tissue, rat lymphonode tissue.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activation B7-1 antigen; B-lymphocyte activation antigen B7; B7 protein; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80-like; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; s1CD80; s2CD80; sCD 80; sCD80; soluble CD 80; soluble CD80; transmembrane protein B1 precursor; TS/A-1; unnamed protein product
Gene Aliases: B7; B7-1; B7.1; B71; BB1; Cd28l; CD28LG; CD28LG1; LAB7; Ly-53; Ly53; MIC17; TSA1
UniProt ID: (Human) P33681, (Mouse) Q00609
Entrez Gene ID: (Human) 941, (Mouse) 12519, (Rat) 25408
immune system process
positive regulation of immune system process
negative regulation of T cell mediated immunity
immune response
cell surface receptor signaling pathway
T cell costimulation
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of T cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
regulation of T cell activation
negative regulation of T cell activation
cellular response to lipopolysaccharide
regulation of leukocyte cell-cell adhesion
positive regulation of signal transduction
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interleukin-2 production
intracellular signal transduction
positive regulation of T-helper 1 cell differentiation
positive regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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