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Invitrogen

DDB1 Polyclonal Antibody

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DDB1 Polyclonal Antibody

Product Details

PA5-79142

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (IHC)

1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL once reconstituted

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746258

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human HEK293 whole cell, human K562 whole cell, human Raji whole cell, human CACO-2 whole cell, human THP-1 whole cell, rat brain tissue, rat kidney tissue, rat C6 whole cell, mouse brain tissue, mouse kidney tissue, mouse testis tissue. IHC: Mouse Liver tissue, Rat Liver tissue, Human Intestinal Cancer tissue IHC-F: human placenta tissue. ICC/IF: A431 cell, A549 Cell, PC-3 Cell, SMMC-7721 Cell. Flow: 293T cell.

Target Information

The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: damage-specific DNA binding protein 1, 127kDa; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; unnamed protein product; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; xeroderma pigmentosum group E-complementing protein; XPE-binding factor

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Gene Aliases: 127kDa; DDBA; p127-Ddb1; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; XPE-BF

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UniProt ID: (Human) Q16531, (Mouse) Q3U1J4

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Entrez Gene ID: (Human) 1642, (Rat) 64470, (Mouse) 13194

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Function(s)
nucleic acid binding DNA binding damaged DNA binding protein binding protein binding, bridging macromolecular complex binding WD40-repeat domain binding cullin family protein binding ubiquitin ligase complex scaffold activity
Process(es)
DNA repair ubiquitin-dependent protein catabolic process cellular response to DNA damage stimulus spindle assembly involved in female meiosis proteasomal protein catabolic process Wnt signaling pathway protein ubiquitination viral release from host cell cellular response to UV regulation of circadian rhythm negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process epigenetic programming in the zygotic pronuclei positive regulation of viral genome replication positive regulation of gluconeogenesis positive regulation of protein catabolic process positive regulation by virus of viral protein levels in host cell interaction with symbiont UV-damage excision repair regulation of mitotic cell cycle phase transition nucleotide-excision repair spermatogenesis cell proliferation regulation of autophagy regulation of DNA-dependent DNA replication initiation replication fork processing regulation of natural killer cell activation regulation of gene expression, epigenetic regulation of cell proliferation regulation of apoptotic process regulation of embryonic development regulation of gene silencing by miRNA regulation of cellular response to stress regulation of cell cycle phase transition regulation of mitotic cytokinesis negative regulation of adipose tissue development regulation of stem cell population maintenance negative regulation of protein kinase B signaling type I interferon signaling pathway base-excision repair, AP site formation via deaminated base removal
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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