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Invitrogen
DLD Polyclonal Antibody
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FIGURE: 1 / 6
DLD Antibody (PA5-79157) in ICC/IF
Immunocytochemistry analysis of DLD using anti-DLD antibody (Product # PA5-79157) . DLD was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DLD antibody (Product # PA5-79157) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualiz... View More
Product Details
PA5-79157
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DLD recombinant protein (Position: K300-F509).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell, human U251 whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue. IHC: human intestinal cancer tissue. ICC/IF: MCF-7 cell. Flow: A549 cell.
Target Information
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: branched chain alpha-keto acid dehydrogenase complex subunit E3; diaphorase; Dihydrolipoamide dehydrogenase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex); dihydrolipoamide dehydrogenase precursor; Dihydrolipoyl dehydrogenase, mitochondrial; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; epididymis secretory sperm binding protein; Glycine cleavage system L protein; glycine cleavage system protein L; lipoamide dehydrogenase; lipoamide dehydrogenase precursor old gene name 'LAD'; lipoamide reductase; lipoyl dehydrogenase; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PH; PHE 3; pyruvate dehydrogenase complex subunit E3, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, glycine cleavage system protein L; unnamed protein product
Gene Aliases: DLD; DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3
UniProt ID: (Human) P09622, (Rat) Q6P6R2, (Mouse) O08749
Entrez Gene ID: (Human) 1738, (Rat) 298942, (Mouse) 13382
dihydrolipoyl dehydrogenase activity
protein binding
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
flavin adenine dinucleotide binding
mercury (II) reductase activity
lipoamide binding
mercury ion binding
NADP binding
NAD binding
oxidoreductase
acetyl-CoA biosynthetic process from pyruvate
pyruvate metabolic process
2-oxoglutarate metabolic process
mitochondrial electron transport, NADH to ubiquinone
proteolysis
gastrulation
branched-chain amino acid catabolic process
regulation of membrane potential
sperm capacitation
2-oxoglutarate decarboxylation to succinyl-CoA
branched-chain alpha-keto acid decarboxylation to branched-chain acyl-CoA
aging
lipoate metabolic process
cell redox homeostasis
detoxification of mercury ion
dihydrolipoamide metabolic process
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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