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Invitrogen
MCAK Polyclonal Antibody
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FIGURE: 1 / 6
MCAK Antibody (PA5-79554) in ICC/IF
Immunocytochemistry analysis of MCAK using anti-MCAK antibody (Product # PA5-79554) . MCAK was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MCAK antibody (Product # PA5-79554) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-79554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MCAK recombinant protein (Position: G531-Q725).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell. IHC: Mouse Testis Tissue, Rat Testis Tissue, Rat Thymus Tissue. ICC/IF: U20S cell. Flow: K562 cell.
Target Information
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HsMCAK; KIF2C; kinesin-like 6; kinesin-like protein 6; kinesin-related protein 2; mitotic centromere-associated kinesin; RP11-269F19.1; testis tissue sperm-binding protein Li 68n; TS-MSAK; unnamed protein product
Gene Aliases: 4930402F02Rik; CT139; ESTM5; KNSL6; KRP2; MCAK
UniProt ID: (Human) Q99661, (Mouse) Q922S8
Entrez Gene ID: (Human) 11004, (Mouse) 73804, (Rat) 171529
microtubule-based movement
microtubule depolymerization
mitotic metaphase plate congression
establishment or maintenance of microtubule cytoskeleton polarity
metaphase plate congression
attachment of mitotic spindle microtubules to kinetochore
regulation of chromosome segregation
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
postsynaptic cytoskeleton organization
mitotic sister chromatid segregation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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