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Invitrogen
TRAF3 Polyclonal Antibody
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FIGURE: 1 / 1
TRAF3 Antibody (PA5-80180) in WB
Western blot analysis of TRAF3 in Lane 1: human K562 cell lysate, Lane 2: rat brain tissue lysate, Lane 3: rat PC-12 cell lysate, Lane 4: mouse brain tissue lysate, Lane 5: mouse HEPA1-6 cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with TRAF3 polyclo... View More
Product Details
PA5-80180
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human TRAF3 recombinant protein (Position: F298-Q551).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 cell, rat brain tissue, rat PC-12 cell, mouse brain tissue, mouse HEPA1-6 cell.
Target Information
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e. g. RANK, CD30, CD40, etc. ) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF1; LAP1; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; TNF receptor-associated factor 3; TRAFAMN; unnamed protein product
Gene Aliases: AI528849; amn; CAP-1; CAP1; CD40bp; CRAF1; IIAE5; IMD132A; IMD132B; LAP1; RNF118; T-BAM; TRAF3; TRAFAMN
UniProt ID: (Human) Q13114, (Mouse) Q60803
Entrez Gene ID: (Human) 7187, (Mouse) 22031, (Rat) 362788
ubiquitin-protein transferase activity
tumor necrosis factor receptor binding
protein binding
zinc ion binding
transferase activity
protein kinase binding
protein phosphatase binding
ubiquitin protein ligase binding
thioesterase binding
signaling adaptor activity
identical protein binding
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
scaffold/adaptor protein
regulation of cytokine production
toll-like receptor signaling pathway
immune system process
apoptotic process
signal transduction
cell surface receptor signaling pathway
Toll signaling pathway
mRNA transcription
regulation of proteolysis
negative regulation of NF-kappaB transcription factor activity
regulation of type I interferon production
positive regulation of type I interferon production
regulation of interferon-beta production
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of defense response to virus
defense response to virus
type I interferon signaling pathway
antiviral innate immune response
protein ubiquitination
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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