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Invitrogen
FOXJ1 Polyclonal Antibody
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FIGURE: 1 / 2
FOXJ1 Antibody (PA5-80644) in WB
Western Blot using FOXJ1 Polyclonal Antibody (Product # PA5-80644) at 1:500 dilution. Lane A: A549 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 49 kDa.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-80644
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human FOXJ1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human FOXJ1.
Target Information
Forkhead-box J1 (FOXJ1) is a 421-amino acid transcription factor that suppresses T cell activity and spontaneous autoimmunity, through the repression of NF B activity. FOXJ1 also inhibits the humoral immune response in B cells. FOXJ1 deficiency in B cells results in spontaneous and accentuated germinal center formation and is implicated in the development of pathogenic autoantibodies and accentuated responses to immunizations. Abnormal expression of FOXJ1 may be associated with autoimmune diseases and/or other inflammatory diseases. FOXJ1 is also required for cilia formation and left-right axis determination because it increases calpastatin expression, a protein necessary for the ability of basal bodies to anchor to the apical cytoskeleton. FOXJ1 expression may function as an early marker of epithelial cell differentiation, recovery, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Forkhead box protein J1; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; MGC35202
Gene Aliases: FKHL13; FOXJ1; HFH4
Entrez Gene ID: (Human) 2302
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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