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Invitrogen
SIN3A Polyclonal Antibody
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FIGURE: 1 / 4
SIN3A Antibody (PA5-81022) in ICC/IF
Immunofluorescence staining of SIN3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SIN3A Polyclonal Antibody (Product # PA5-81022, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-81022
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Sin3a
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has specificity for Human Sin3a.
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: histone deacetylase complex subunit Sin3a; Mammalian Sin3A; similar to yeast SIN3; SIN; SIN3 homolog A, transcription regulator; transcriptional co-repressor Sin3A; transcriptional corepressor Sin3a; transcriptional regulator, SIN3A; unnamed protein product
Gene Aliases: CHR15DELq24; DEL15Q24; WITKOS
UniProt ID: (Human) Q96ST3
Entrez Gene ID: (Human) 25942
negative regulation of transcription from RNA polymerase II promoter
activation of innate immune response
positive regulation of defense response to virus by host
regulation of transcription, DNA-templated
regulation of hormone levels
cerebral cortex neuron differentiation
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of axon extension
heterochromatin assembly
negative regulation of circadian rhythm
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
type I interferon signaling pathway
negative regulation of transcription initiation from RNA polymerase II promoter
cellular response to tert-butyl hydroperoxide
negative regulation of protein localization to nucleus
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
cellular response to dopamine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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