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Invitrogen
PTBP1 Polyclonal Antibody
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FIGURE: 1 / 9
PTBP1 Antibody (PA5-81297) in ICC/IF
Immunofluorescence staining of PTBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PTBP1 Polyclonal Antibody (Product # PA5-81297, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Product Details
PA5-81297
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human PTBP1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human PTBP1.
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; MGC10830; MGC8461; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); Polypyrimidine tract-binding protein 1; PTB; RNA-binding protein
Gene Aliases: HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; PTBP1
UniProt ID: (Human) P26599
Entrez Gene ID: (Human) 5725
regulation of alternative mRNA splicing, via spliceosome
mRNA processing
RNA splicing
gene expression
neurogenesis
negative regulation of RNA splicing
regulation of RNA splicing
regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of mRNA splicing, via spliceosome
negative regulation of muscle cell differentiation
positive regulation of calcineurin-NFAT signaling cascade
IRES-dependent viral translational initiation
negative regulation of mRNA metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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