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Invitrogen
MIF Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-82117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MIF. Recombinant protein control fragment (Product #RP-91534).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FIVNTNVPRA SVPDGFLSEL TQQLAQATGK PPQYIAVHVV PDQLMAFGGS SEPCALCSLH SIGKIGGAQN RSYSKLLCGL LAERLRISPD RVYINYYDMN AANVGWNN
Highest antigen sequence indentity to the following orthologs: Rat - 90%, Mouse - 89%.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: delayed early response protein 6; epididymis secretory sperm binding protein; glutathione-binding 13 kDa protein; glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; MIF; migration inhibitory factor; phenylpyruvate tautomerase; unnamed protein product
Gene Aliases: DER6; GIF; GLIF; MMIF
UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904
Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683
prostaglandin biosynthetic process
positive regulation of cytokine production
positive regulation of adaptive immune response
negative regulation of mature B cell apoptotic process
cell surface receptor signaling pathway
positive regulation of cell proliferation
negative regulation of gene expression
negative regulation of macrophage chemotaxis
DNA damage response, signal transduction by p53 class mediator
negative regulation of cell migration
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of prostaglandin secretion
positive regulation of tumor necrosis factor production
negative regulation of myeloid cell apoptotic process
regulation of cell proliferation
positive regulation of phosphorylation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of innate immune response
positive regulation of fibroblast proliferation
positive chemotaxis
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
regulation of cellular response to stress
positive regulation of arachidonic acid secretion
cellular senescence
positive regulation of cAMP/PKA signal transduction
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
negative regulation of cellular senescence
carboxylic acid metabolic process
regulation of macrophage activation
hair follicle development
brain renin-angiotensin system
positive regulation of acute inflammatory response
spermatogenesis
response to xenobiotic stimulus
response to mechanical stimulus
response to hormone
positive regulation of smooth muscle cell migration
response to estradiol
response to lipopolysaccharide
response to progesterone
response to menaquinone
response to insulin
response to vitamin E
drinking behavior
positive regulation of potassium ion transport
skin development
positive regulation of glycolytic process
positive regulation of glucose import
positive regulation of axon regeneration
positive regulation of immune response
response to glucocorticoid
cellular response to hydrogen peroxide
cellular response to hypoxia
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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