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Invitrogen
Epo Polyclonal Antibody
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FIGURE: 1 / 4
Epo Antibody (PA5-85107) in IHC (P)
Epo Polyclonal Antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Epo Polyclonal Antibody (Product # PA5-85107) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-85107
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human EPO. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.68 mg/mL
Amount
68 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Rat (80%), Dog (80%), Cat (82%), Pig (81%), Rabbit (80%), Sheep (81%), Rhesus Monkey (89%), Bovine (80%).
Positive Control: rPT EPO.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epoetin; MGC138142; unnamed protein product
Gene Aliases: DBAL; ECYT5; EP; MVCD2
UniProt ID: (Human) P01588, (Rat) P29676
Entrez Gene ID: (Human) 2056, (Rat) 24335
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
response to stress
acute-phase response
signal transduction
response to nutrient
blood circulation
positive regulation of cell proliferation
response to salt stress
negative regulation of calcium ion transport into cytosol
positive regulation of neuron projection development
erythrocyte differentiation
response to lipopolysaccharide
response to vitamin A
response to testosterone
erythropoietin-mediated signaling pathway
positive regulation of activated T cell proliferation
negative regulation of apoptotic process
response to estrogen
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of Ras protein signal transduction
response to axon injury
response to electrical stimulus
response to hyperoxia
positive regulation of ERK1 and ERK2 cascade
response to interleukin-1
cellular hyperosmotic response
response to dexamethasone
receptor signaling pathway via STAT
negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
negative regulation of erythrocyte apoptotic process
regulation of transcription from RNA polymerase II promoter
apoptotic process
embryo implantation
peptidyl-serine phosphorylation
negative regulation of myeloid cell apoptotic process
hemoglobin biosynthetic process
positive regulation of protein kinase activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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