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Invitrogen
BRD4 Polyclonal Antibody
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FIGURE: 1 / 12
BRD4 Antibody (PA5-85662) in ICC/IF
Immunofluorescence analysis of Bromodomain-containing protein 4 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BRD4 Polyclonal Antibody (Product # PA5-85662) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-85662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human BRD4 . The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.48 mg/mL
Amount
48 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Pig (98%).
Positive Control: 293T nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BRD4-NUT FUSION; bromodomain-containing 5; chromatin associated phosphoprotein; chromosome-associated protein; female sterile homeotic related gene 4; fsh-like protein; HUNKI; mitotic chromosome-associated protein; unnamed protein product
Gene Aliases: Brd5; CAP; CDLS6; FSHRG4; HUNK1; HUNKI; MCAP; WI-11513
UniProt ID: (Human) O60885, (Mouse) Q9ESU6
Entrez Gene ID: (Human) 23476, (Rat) 362844, (Mouse) 57261
transcription regulatory region sequence-specific DNA binding
p53 binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
protein serine/threonine kinase activity
protein binding
RNA polymerase II carboxy-terminal domain kinase activity
enzyme binding
protein binding, bridging
histone binding
RNA polymerase II C-terminal domain binding
P-TEFb complex binding
histone H4 reader activity
histone H4K12ac reader activity
histone H4K5ac reader activity
histone H4K16ac reader activity
histone H4K8ac reader activity
histone H3K9ac reader activity
histone H3K27ac reader activity
DNA binding
GTP-dependent protein kinase activity
histone reader activity
inner cell mass cell proliferation
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
cellular response to DNA damage stimulus
chromosome segregation
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
regulation of inflammatory response
cellular response to glucose stimulus
negative regulation of DNA damage checkpoint
positive regulation of T-helper 17 cell lineage commitment
chromatin organization
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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