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Invitrogen
Caspase 8 Polyclonal Antibody
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FIGURE: 1 / 2
Caspase 8 Antibody (PA5-86072) in WB
Western blot analysis of Caspase 8 in Lane 1: sp2/0 cell lysate treated with colchicine (0.2 ng/mL, 15mins), Lane 2: H9C2 cell lysate with colchicine (0.2 ng/mL, 15mins). Sample was incubated with Caspase 8 polyclonal antibody (Product # PA5-86072) using a 1:500 dilution.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-86072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 310-362 of Human Caspase-8.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: apoptotic cysteine protease; apoptotic protease Mch-5; Casp8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8 precursor; caspase-8s; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; flice; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581; unnamed protein product
Gene Aliases: ALPS2B; CAP4; CASP-8; FLICE; MACH; MCH5
UniProt ID: (Human) Q14790, (Mouse) O89110, (Rat) Q9JHX4
Entrez Gene ID: (Human) 841, (Mouse) 12370, (Rat) 64044
endopeptidase activity
cysteine-type endopeptidase activity
death receptor binding
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
ubiquitin protein ligase binding
death effector domain binding
identical protein binding
macromolecular complex binding
scaffold protein binding
tumor necrosis factor receptor binding
angiogenesis
regulation of cytokine production
neural tube formation
regulation of immune system process
proteolysis
apoptotic process
heart development
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of signal transduction
protein processing
cell differentiation
protein catabolic process
macrophage differentiation
positive regulation of cell migration
positive regulation of interleukin-1 beta production
response to tumor necrosis factor
TRAIL-activated apoptotic signaling pathway
regulation of apoptotic process
chordate embryonic development
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
regulation of innate immune response
response to ethanol
positive regulation of macrophage differentiation
positive regulation of proteolysis
animal organ development
cardiac muscle tissue development
protein maturation
negative regulation of necroptotic process
regulation of thymocyte apoptotic process
pyroptosis
cellular response to mechanical stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
execution phase of apoptosis
self proteolysis
hepatocyte apoptotic process
positive regulation of pyroptotic inflammatory response
positive regulation of execution phase of apoptosis
regulation of apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
response to auditory stimulus
response to cobalt ion
response to estradiol
response to lipopolysaccharide
response to anesthetic
regulation of tumor necrosis factor-mediated signaling pathway
natural killer cell activation
T cell activation
B cell activation
syncytiotrophoblast cell differentiation involved in labyrinthine layer development
death-inducing signaling complex assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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