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Invitrogen
TCF12 Polyclonal Antibody
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FIGURE: 1 / 2
TCF12 Antibody (PA5-86979) in WB
Western blot analysis of TCF12 in Mouse thymus. Samples were incubated with TCF12 polyclonal antibody (Product # PA5-86979) using a dilution of 1:430 followed by HRP Goat Anti-Rabbit IgG at 1:10,000 dilution. Lysates/proteins: 25 µg per Lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-86979
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein of human TCF12.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class A helix-loop-helix transcription factor ME1; class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; helix-loop-helix transcription factor 4; salivary-specific cAMP response element-binding protein alpha; SCBP-alpha; TCF-12; transcription factor HTF-4; unnamed protein product
Gene Aliases: A130037E08Rik; ALF1; bHLHb20; CRS3; HEB; HEBAlt; HH26; HsT17266; HTF-4; HTF4; ME1; p64; REB; SCBP alpha; SCBPA; TCF-12
UniProt ID: (Human) Q99081, (Rat) P51514, (Mouse) Q61286
Entrez Gene ID: (Human) 6938, (Rat) 25720, (Mouse) 21406
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
cAMP response element binding
bHLH transcription factor binding
SMAD binding
protein dimerization activity
HMG box domain binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
DNA binding
protein heterodimerization activity
E-box binding
regulation of transcription from RNA polymerase II promoter
immune response
nervous system development
muscle organ development
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to gonadotropin-releasing hormone
positive regulation of gene expression
positive regulation of neuron differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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