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Invitrogen
Calcineurin B Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-87531
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_0009361)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.93 mg/mL
Amount
193 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV; Positive Samples: Mouse brain, Mouse spleen, Mouse lung, Rat brain, SH-SY5Y, SW480, HL-60, MCF7, Rat testis; Cellular Location: Cell membrane, Cytoplasm, Lipid-anchor, Membrane, cytosol, sarcolemma
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALNB1; calcineurin B, type I (19kDa); PP2B beta 1; PPP3R1; protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 regulatory subunit B alpha; unnamed protein product
Gene Aliases: CALNB1; CaNB1; CNB; CNB1; MCIP1
UniProt ID: (Human) P63098, (Rat) P63100, (Mouse) Q63810
Entrez Gene ID: (Human) 5534, (Rat) 29748, (Mouse) 19058
calcineurin-NFAT signaling cascade
positive regulation of transcription from RNA polymerase II promoter
positive regulation of calcineurin-NFAT signaling cascade
regulation of synaptic vesicle cycle
regulation of postsynaptic neurotransmitter receptor internalization
postsynaptic modulation of chemical synaptic transmission
positive regulation of calcium ion import across plasma membrane
negative regulation of calcium ion import across plasma membrane
patterning of blood vessels
epithelial to mesenchymal transition
protein dephosphorylation
protein import into nucleus
heart development
Schwann cell development
myelination in peripheral nervous system
protein localization to nucleus
lung epithelial cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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