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Invitrogen
AP3M1 Polyclonal Antibody
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FIGURE: 1 / 1
AP3M1 Antibody (PA5-87877) in WB
Western blot analysis of AP3M1 in extracts of various cell lines. Samples were incubated with AP3M1 Polyclonal antibody (Product # PA5-87877) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-87877
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 219-418 of human AP3M1 (NP_0362271)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.78 mg/mL
Amount
178 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SLSFMNPRLL DDVSFHPCIR FKRWESERVL SFIPPDGNFR LISYRVSSQN LVAIPVYVKH SISFKENSSC GRFDITIGPK QNMGKTIEGI TVTVHMPKVV LNMNLTPTQG SYTFDPVTKV LTWDVGKITP QKLPSLKGLV NLQSGAPKPE ENPSLNIQFK IQQLAISGLK VNRLDMYGEK YKPFKGVKYV TKAGKFQVRT; Positive Samples: 22Rv1, Mouse liver, Mouse kidney, Rat spinal cord, Rat liver; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle membrane, Golgi apparatus, Peripheral membrane protein
Target Information
The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adapter-related protein complex 3 mu-1 subunit; adapter-related protein complex 3 subunit mu-1; adaptor related protein complex 3 mu 1 subunit; adaptor-related protein complex 3 subunit mu-1; adaptor-related protein complex 3, mu 1 subunit; adaptor-related protein complex AP-3, mu 1 subunit; AP-3 adapter complex mu3A subunit; AP-3 adaptor complex mu3A subunit; ap3; clathrin adaptor complex AP3, mu-3A subunit; clathrin adaptor protein mu3A; clathrin assembly protein assembly protein complex 1 medium chain homolog 1; clathrin assembly protein assembly protein complex 3 mu-1 medium chain; clathrin coat assembly protein AP47 homolog 1; clathrin coat-associated protein AP47 homolog 1; clathrin-associated adaptor protein homolog (p47A); golgi adaptor AP-1 47 kDa protein homolog 1; HA1 47 kDa subunit homolog 1; medium chain; Mu-adaptin 3A; mu3A-adaptin; unnamed protein product
Gene Aliases: 1200013D09Rik; P47a
UniProt ID: (Human) Q9Y2T2, (Mouse) Q9JKC8
Entrez Gene ID: (Human) 26985, (Rat) 171126, (Mouse) 55946
intracellular protein transport
Golgi to vacuole transport
endocytosis
anterograde axonal transport
vesicle-mediated transport
anterograde synaptic vesicle transport
postsynaptic neurotransmitter receptor internalization
protein targeting to lysosome
clathrin-coated vesicle cargo loading, AP-3-mediated
platelet dense granule organization
melanosome assembly
vesicle transport along microtubule
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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