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Invitrogen
FLT3 Polyclonal Antibody
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FIGURE: 1 / 1
FLT3 Antibody (PA5-88560) in WB
Western blot analysis of FLT3 in extracts of various cell lines. Samples were incubated with FLT3 Polyclonal antibody (Product # PA5-88560) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-88560
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.61 mg/mL
Amount
161 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse liver, Rat liver; Cellular Location: Endoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein
Immunogen sequence: NYLRSKREKF HRTWTEIFKE HNFSFYPTFQ SHPNSSMPGS REVQIHPDSD QISGLHGNSF HSEDEIEYEN QKRLEEEEDL NVLTFEDLLC FAYQVAKGME
Target Information
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fetal liver kinase 2; FL cytokine receptor; flk-2; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; OTTHUMP00000214829; RP11-153M24.3; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; unnamed protein product
Gene Aliases: B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl
UniProt ID: (Mouse) Q00342
Entrez Gene ID: (Mouse) 14255, (Rat) 140635
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
transmembrane signaling receptor activity
cytokine receptor activity
protein binding
ATP binding
growth factor binding
ubiquitin protein ligase binding
glucocorticoid receptor binding
phosphatidylinositol 3-kinase binding
macromolecular complex binding
nucleotide binding
leukocyte homeostasis
positive regulation of protein phosphorylation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
pro-B cell differentiation
pro-T cell differentiation
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
post-embryonic development
cell migration
cytokine-mediated signaling pathway
antigen processing and presentation
hemopoiesis
lymphocyte differentiation
B cell differentiation
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
common myeloid progenitor cell proliferation
dendritic cell homeostasis
positive regulation of multicellular organism growth
negative regulation of B cell differentiation
lymphocyte proliferation
protein autophosphorylation
lymph node development
spleen development
homeostasis of number of cells within a tissue
regulation of developmental process
cellular response to cytokine stimulus
dendritic cell differentiation
cellular response to virus
cellular response to glucocorticoid stimulus
liver regeneration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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