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Invitrogen
Nkx2.5 Polyclonal Antibody
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FIGURE: 1 / 1
Nkx2.5 Antibody (PA5-88705) in WB
Western blot analysis of Nkx2.5 in extracts of various cell lines. Samples were incubated with Nkx2.5 Polyclonal antibody (Product # PA5-88705) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88705
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human NKX2-5 (NP_0043781)
Conjugate
Form
Liquid
Concentration
1.73 mg/mL
Amount
173 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AFKPEAYAGP EAAAPGLPEL RAELGRAPSP AKCASAFPAA PAFYPRAYSD PDPAKDPRAE KKELCALQKA VELEKTEADN AERPRARRRR KPRVLFSQAQ V; Positive Samples: Mouse heart, Rat heart; Cellular Location: Nucleus
Target Information
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac-specific homeobox; Drosophila NK2 transcription factor related, locus 5; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5; Nkx-25; rNKx-2.5
Gene Aliases: Csx; Nkx-2.5; Nkx2-5; Nkx2.5; Nkx2e; tinman
UniProt ID: (Rat) O35767, (Mouse) P42582
Entrez Gene ID: (Rat) 114109, (Mouse) 18091
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription cofactor activity
transcriptional activator activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
transcription factor binding
serum response element binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
vasculogenesis
heart looping
heart morphogenesis
outflow tract septum morphogenesis
outflow tract morphogenesis
cardiac conduction system development
atrioventricular node development
bundle of His development
Purkinje myocyte differentiation
cardiac ventricle morphogenesis
cardiac ventricle formation
right ventricular cardiac muscle tissue morphogenesis
ventricular trabecula myocardium morphogenesis
apoptotic process involved in heart morphogenesis
septum secundum development
proepicardium development
pulmonary myocardium development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
heart development
adult heart development
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
positive regulation of transcription via serum response element binding
positive regulation of sodium ion transport
negative regulation of myotube differentiation
hemopoiesis
cell differentiation
BMP signaling pathway
thyroid gland development
response to estradiol
embryonic heart tube development
negative regulation of apoptotic process
sarcomere organization
positive regulation of neuron differentiation
positive regulation of heart contraction
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
spleen development
cardiac muscle tissue development
positive regulation of cardioblast differentiation
ventricular cardiac myofibril assembly
cardiac muscle cell differentiation
cardiac muscle tissue morphogenesis
regulation of cardiac muscle contraction
pharyngeal system development
cardiac muscle cell proliferation
regulation of cardiac muscle cell proliferation
heart contraction
cardiac muscle contraction
canonical Wnt signaling pathway
positive regulation of transcription initiation from RNA polymerase II promoter
heart trabecula formation
ventricular septum morphogenesis
atrial septum morphogenesis
atrioventricular node cell development
atrioventricular node cell fate commitment
embryonic heart tube left/right pattern formation
cellular response to growth factor stimulus
cardiovascular system development
negative regulation of canonical Wnt signaling pathway
positive regulation of voltage-gated calcium channel activity
regulation of transcription, DNA-templated
multicellular organism development
endoderm development
tongue development
atrial cardiac muscle cell development
ventricular cardiac muscle cell development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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