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Antibody Testing Data (3)

TRIM27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

TRIM27 Antibody (PA5-90911) in IHC (P)

Immunohistochemistry analysis of TRIM27 in paraffin-embedded mouse kidney. Samples were incubated with TRIM27 Polyclonal antibody (Product # PA5-90911) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image TRIM27 Polyclonal Antibody

Product Details

PA5-90911

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TRIM27 (NP_0065011)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.79 mg/mL

79 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2806287

Product Specific Information

Immunogen sequence: MASGSVAECL QQETTCPVCL QYFAEPMMLD CGHNICCACL ARCWGTAETN VSCPQCRETF PQRHMRPNRH LANVTQLVKQ LRTERPSGPG GEMGVCEKHR EPLKLYCEED QMPICVVCDR SREHRGHSVL PLEEAVEGFK EQIQNQLDHL KRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL; Positive Samples: HeLa, HepG2, Raji, SH-SY5Y; Cellular Location: Cytoplasm, Early endosome, Nucleus, PML body

Target Information

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ret finger protein; ret finger protein 1; RFP transforming protein; RING finger protein 76; RING-type E3 ubiquitin transferase TRIM27; tripartite motif protein 27; tripartite motif protein TRIM27; tripartite motif-containing protein 27; unnamed protein product

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Gene Aliases: Gm19403; RFP; Rfp1; RNF76

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UniProt ID: (Human) P14373, (Mouse) Q62158

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Entrez Gene ID: (Human) 5987, (Mouse) 19720, (Rat) 291171

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Function(s)

nucleic acid binding transcription coactivator activity ubiquitin-protein transferase activity protein binding zinc ion binding SUMO transferase activity identical protein binding metal ion binding ubiquitin protein ligase activity phosphatidylinositol 3-kinase inhibitor activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter negative regulation of autophagy positive regulation of autophagy regulation of tumor necrosis factor-mediated signaling pathway protein ubiquitination negative regulation of type I interferon production positive regulation of type I interferon production negative regulation of viral transcription Arp2/3 complex-mediated actin nucleation retrograde transport, endosome to Golgi negative regulation by host of viral release from host cell innate immune response negative regulation of gene expression, epigenetic positive regulation of transcription, DNA-templated positive regulation of actin nucleation protein K63-linked ubiquitination antiviral innate immune response spermatogenesis protein sumoylation

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TRIM27 Polyclonal Antibody

TRIM27 Antibody (PA5-90911) in IHC (P)

Product Details

PA5-90911

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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