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Invitrogen
CD200 Polyclonal Antibody
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FIGURE: 1 / 1
CD200 Antibody (PA5-93052) in WB
Western blot analysis of CD200 in extracts of various cell lines. Samples were incubated with CD200 Polyclonal antibody (Product # PA5-93052) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-93052
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-240 of human CD200 (NP_0059354)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.51 mg/mL
Amount
151 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AQVQVVTQDE REQLYTPASL KCSLQNAQEA LIVTWQKKKA VSPENMVTFS ENHGVVIQPA YKDKINITQL GLQNSTITFW NITLEDEGCY MCLFNTFGFG KISGTACLTV YVQPIVSLHY KFSEDHLNIT CSATARPAPM VFWKVPRSGI ENSTVTLSHP NGTTSVTSIL HIKDPKNQVG KEVICQVLHL GTVTDFKQTV NKGYWFSVPL L; Positive Samples: Mouse brain, Mouse heart, Rat brain; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; CD200 antigen; cell adhesion molecule; extracellular region; cell surface protein (thymocyte antigen identified by monoclonal antibody MRC-OX2); MRC OX-2 antigen; unnamed protein product
Gene Aliases: Cspmo2; MOX1; MOX2; MRC; MRCOX2; OX-2; OX2
UniProt ID: (Human) P41217
Entrez Gene ID: (Human) 4345, (Rat) 24560, (Mouse) 17470
negative regulation of cell proliferation
negative regulation of interleukin-6 production
heterotypic cell-cell adhesion
negative regulation of macrophage activation
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
regulation of immune response
cellular response to lipopolysaccharide
cellular response to interferon-gamma
positive regulation of transforming growth factor beta production
cell-cell adhesion
regulation of neuroinflammatory response
negative regulation of neuroinflammatory response
negative regulation of matrix metallopeptidase secretion
negative regulation of macrophage migration
negative regulation of T cell migration
negative regulation of leukocyte activation
regulation of macrophage activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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