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Invitrogen
TAF1C Polyclonal Antibody
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FIGURE: 1 / 1
TAF1C Antibody (PA5-93203) in WB
Western blot analysis of TAF1C in extracts of various cell lines. Samples were incubated with TAF1C Polyclonal antibody (Product # PA5-93203) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93203
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 70-320 of human TAF1C (NP_0012300851)
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Amount
30 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PPLIDPWDPG LTARDLLFRG GYRYRKRPRV VLDVTEQISR FLLDHGDVAF APLGKLMLEN FKLEGAGSRT KKKTVVSVKK LLQDLGGHQP WGCPWAYLSN RQRRFSILGG PILGTSVASH LAELLHEELV LRWEQLLLDE ACTGGALAWV PGRTPQFGQL VYPAGGAQDR LHFQEVVLTP GDNPQFLGKP GRIQLQGPVR QVVTCTVQGE TLLAVRSDYH CAVWKFGKQW QPTLLQAMQV EKGATGISLS P; Positive Samples: 293T, MCF7, Mouse thymus; Cellular Location: Nucleus
Target Information
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. TAF1C is the largest SL1-specific TAF. Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA polymerase I-specific TBP-associated factor 110 kDa; RNA polymerase I-specific TBP-associated factor 95 kDa; SL1, 110kD subunit; TAFI110; TAFI95; TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kD; TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa; TATA box binding protein (Tbp)-associated factor, RNA polymerase I, C; transcription factor TAFI95; TATA box-binding protein-associated factor 1C; TATA box-binding protein-associated factor RNA polymerase I subunit C; TATA box-binding protein-associated factor, RNA polymerase I, subunit C; TATA-box binding protein associated factor, RNA polymerase I, C; TBP-associated factor 1C; TBP-associated factor RNA polymerase I 95 kDa; transcription factor SL1; transcription factor TAFI95; Transcription initiation factor SL1/TIF-IB subunit C; unnamed protein product
Gene Aliases: MGC:39976; mTAFI95; SL1; TAF1C; TAFI110; TAFI95
UniProt ID: (Human) Q15572, (Mouse) Q6PDZ2, (Rat) Q6P773
Entrez Gene ID: (Human) 9013, (Mouse) 21341, (Rat) 361420
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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