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Invitrogen

BDNF Polyclonal Antibody

3 Published Figures
5 References
View all (49) BDNF antibodies

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BDNF Polyclonal Antibody

Product Details

PA5-95183

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
View 2 publications 2 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human BDNF recombinant protein (Position: H129-R247).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL once reconstituted

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806988

Product Specific Information

Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: recombinant human BDNF protein. IHC: mouse brain tissue, rat brain tissue .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: abrineurin; BDNF; brain derived neurothrophic factor; brain-derived neurotrophic factor; growth factor; BDNF; H-BDNF; hBDNF1; hBDNF2A; hBDNF2B; hBDNF2C; hBDNF3; hBDNF4; hBDNF5; hBDNF6A; hBDNF6B; hBDNF7; mBDNF6a; mBDNF6b; neurotrophic factor; neurotrophin; proBDNF; unnamed protein product

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Gene Aliases: ANON2; BULN2

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UniProt ID: (Human) P23560, (Mouse) P21237, (Rat) P23363

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Entrez Gene ID: (Human) 627, (Mouse) 12064, (Rat) 24225

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Function(s)
receptor binding nerve growth factor receptor binding protein binding growth factor activity neurotrophin TRKB receptor binding
Process(es)
transmembrane receptor protein tyrosine kinase signaling pathway nervous system development axon guidance synapse assembly circadian rhythm negative regulation of myotube differentiation positive regulation of neuron projection development nerve development brain-derived neurotrophic factor receptor signaling pathway positive regulation of brain-derived neurotrophic factor receptor signaling pathway nerve growth factor signaling pathway negative regulation of neuron apoptotic process collateral sprouting positive regulation of collateral sprouting neuron projection morphogenesis modulation of synaptic transmission positive regulation of synapse assembly trans-synaptic signaling by BDNF, modulating synaptic transmission regulation of protein localization to cell surface negative regulation of apoptotic signaling pathway ureteric bud development behavioral fear response mitochondrial electron transport, NADH to ubiquinone gamma-aminobutyric acid signaling pathway negative regulation of neuroblast proliferation axon target recognition learning or memory learning feeding behavior neuron recognition response to xenobiotic stimulus glutamate secretion dendrite development regulation of axon extension regeneration neuron projection regeneration negative regulation of synaptic transmission, GABAergic positive regulation of peptidyl-serine phosphorylation mechanoreceptor differentiation fear response negative regulation of apoptotic process negative regulation of programmed cell death regulation of neuron apoptotic process positive regulation of neuron apoptotic process positive regulation of neuron differentiation negative regulation of striated muscle tissue development positive regulation of transcription from RNA polymerase II promoter regulation of retinal cell programmed cell death behavioral response to cocaine regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity positive regulation of long-term neuronal synaptic plasticity regulation of short-term neuronal synaptic plasticity regulation of collateral sprouting axon extension positive regulation of axon regeneration inner ear development excitatory postsynaptic potential inhibitory postsynaptic potential taste bud development dendrite extension neuron projection extension conditioned place preference positive regulation of glucocorticoid receptor signaling pathway response to hypoxia chronic inflammatory response response to light stimulus response to light intensity response to hormone response to auditory stimulus response to activity neuron differentiation response to nutrient levels response to food response to vitamin A response to tumor necrosis factor response to potassium ion cellular response to potassium ion response to morphine response to ethanol cognition response to electrical stimulus response to hyperoxia cellular response to tumor necrosis factor cellular response to norepinephrine stimulus response to anesthetic response to L-glutamate response to nerve growth factor cellular response to nerve growth factor stimulus
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